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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP322509BAP
CSB-EP322509BAP
CSB-BP322509BAP
CSB-MP322509BAP
CSB-EP322509BAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spiralin (spi) CSB-YP322510FKO
CSB-BP322510FKO
CSB-MP322510FKO
CSB-EP322510FKO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Methylated-DNA--protein-cysteine methyltransferase, inducible (adaB) CSB-YP322511BRJ
CSB-EP322511BRJ
CSB-BP322511BRJ
CSB-MP322511BRJ
CSB-EP322511BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beet necrotic yellow vein mosaic virus RNA-3 uncharacterized 11.6 kDa protein CSB-YP322512BGA
CSB-EP322512BGA
CSB-BP322512BGA
CSB-MP322512BGA
CSB-EP322512BGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum 18.1 kDa class I heat shock protein (HSP18.1) CSB-YP322513EWE
CSB-BP322513EWE
CSB-MP322513EWE
CSB-EP322513EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Asparagine synthetase, root [glutamine-hydrolyzing] (AS2), partial CSB-YP322514EWE
CSB-EP322514EWE
CSB-BP322514EWE
CSB-MP322514EWE
CSB-EP322514EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermus fervidus DNA-binding protein HMf-2 (hmfB) CSB-YP322515MFF 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Thermoproteus tenax virus 1 Viral protein TPX CSB-YP322516TDP
CSB-EP322516TDP
CSB-BP322516TDP
CSB-MP322516TDP
CSB-EP322516TDP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus tenax virus 1 Uncharacterized 6.5 kDa protein CSB-YP322517TDO
CSB-EP322517TDO
CSB-BP322517TDO
CSB-MP322517TDO
CSB-EP322517TDO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus tenax virus 1 Uncharacterized 15.3 kDa protein CSB-YP322518TDO
CSB-EP322518TDO
CSB-BP322518TDO
CSB-MP322518TDO
CSB-EP322518TDO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus tenax virus 1 Uncharacterized 10.5 kDa protein CSB-YP322519TDO
CSB-EP322519TDO
CSB-BP322519TDO
CSB-MP322519TDO
CSB-EP322519TDO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus tenax virus 1 Uncharacterized 6.0 kDa protein CSB-YP322520TDO
CSB-EP322520TDO
CSB-BP322520TDO
CSB-MP322520TDO
CSB-EP322520TDO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lemna gibba Ribulose bisphosphate carboxylase small chain SSU40B, chloroplastic (SSU40B) CSB-YP322521LDU
CSB-EP322521LDU
CSB-BP322521LDU
CSB-MP322521LDU
CSB-EP322521LDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable nitrate reductase molybdenum cofactor assembly chaperone NarW (narW) CSB-YP322522ENV
CSB-EP322522ENV
CSB-BP322522ENV
CSB-MP322522ENV
CSB-EP322522ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schistosoma mansoni 23 kDa integral membrane protein, partial CSB-YP322523SXQ1
CSB-EP322523SXQ1
CSB-BP322523SXQ1
CSB-MP322523SXQ1
CSB-EP322523SXQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Artemia sp. Extracellular globin-E7 CSB-YP322524AOF
CSB-EP322524AOF
CSB-BP322524AOF
CSB-MP322524AOF
CSB-EP322524AOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein snail homolog Sna (snai1) CSB-YP322525XBE
CSB-EP322525XBE
CSB-BP322525XBE
CSB-MP322525XBE
CSB-EP322525XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Capsule polysaccharide export inner-membrane protein BexB (bexB), partial CSB-YP322526HSZ1
CSB-EP322526HSZ1
CSB-BP322526HSZ1
CSB-MP322526HSZ1
CSB-EP322526HSZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ascaris suum Major pepsin inhibitor 3 (PI-3) CSB-YP322527DOS
CSB-BP322527DOS
CSB-MP322527DOS
CSB-EP322527DOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pesticidal crystal protein cry1Da (cry1Da), partial CSB-YP322528BCU
CSB-EP322528BCU
CSB-BP322528BCU
CSB-MP322528BCU
CSB-EP322528BCU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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