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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Vaccinia virus Serine proteinase inhibitor 3 (SPI-3) CSB-YP321816VAA
CSB-EP321816VAA
CSB-BP321816VAA
CSB-MP321816VAA
CSB-EP321816VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein K4 (K4L) CSB-YP321817VAA
CSB-EP321817VAA
CSB-BP321817VAA
CSB-MP321817VAA
CSB-EP321817VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 8.4 kDa protein (B ORF F) CSB-YP321818VAA
CSB-EP321818VAA
CSB-BP321818VAA
CSB-MP321818VAA
CSB-EP321818VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 8.5 kDa protein (D ORF B) CSB-YP321819VAA
CSB-EP321819VAA
CSB-BP321819VAA
CSB-MP321819VAA
CSB-EP321819VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 7.6 kDa protein (E ORF D) CSB-YP321820VAA
CSB-EP321820VAA
CSB-BP321820VAA
CSB-MP321820VAA
CSB-EP321820VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 9.9 kDa protein (F ORF D) CSB-YP321821VAA
CSB-EP321821VAA
CSB-BP321821VAA
CSB-MP321821VAA
CSB-EP321821VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 8.1 kDa protein (H ORF A) CSB-YP321822VAA
CSB-EP321822VAA
CSB-BP321822VAA
CSB-MP321822VAA
CSB-EP321822VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 9.0 kDa protein (K ORF B) CSB-YP321823VAA
CSB-EP321823VAA
CSB-BP321823VAA
CSB-MP321823VAA
CSB-EP321823VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Indole-3-glycerol phosphate synthase (trpC) CSB-YP321824FFZ
CSB-EP321824FFZ
CSB-BP321824FFZ
CSB-MP321824FFZ
CSB-EP321824FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces olivaceoviridis Alpha-amylase inhibitor Paim-2 CSB-YP321825SPB
CSB-EP321825SPB
CSB-BP321825SPB
CSB-MP321825SPB
CSB-EP321825SPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus megaterium ATP synthase subunit c (atpE), partial CSB-YP321826BQW1
CSB-EP321826BQW1
CSB-BP321826BQW1
CSB-MP321826BQW1
CSB-EP321826BQW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant L-lactate dehydrogenase X (ldhX) CSB-YP321827BBM
CSB-EP321827BBM
CSB-BP321827BBM
CSB-MP321827BBM
CSB-EP321827BBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum Nitrogenase iron protein (nifH) CSB-YP321828RKN
CSB-BP321828RKN
CSB-MP321828RKN
CSB-EP321828RKN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus Protein nifQ (nifQ) CSB-YP321829RLC
CSB-EP321829RLC
CSB-BP321829RLC
CSB-MP321829RLC
CSB-EP321829RLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Early transcription factor 70 kDa subunit (VETFS), partial CSB-YP321830VAA
CSB-EP321830VAA
CSB-BP321830VAA
CSB-MP321830VAA
CSB-EP321830VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein K3 (K3L) CSB-YP321831VAA
CSB-BP321831VAA
CSB-MP321831VAA
CSB-EP321831VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saponaria officinalis Ribosome-inactivating protein saporin-6 (SAP6) CSB-YP321832SCG
CSB-EP321832SCG
CSB-BP321832SCG
CSB-MP321832SCG
CSB-EP321832SCG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Myosin light chain kinase 2, skeletal/cardiac muscle (Mylk2) CSB-YP321833RA
CSB-EP321833RA
CSB-BP321833RA
CSB-MP321833RA
CSB-EP321833RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helianthus annuus Isocitrate lyase CSB-YP321834HUO
CSB-EP321834HUO
CSB-BP321834HUO
CSB-MP321834HUO
CSB-EP321834HUO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Uncharacterized 12.6 kDa protein in mrh-soc intergenic region (y01I) CSB-YP321835EDZ
CSB-EP321835EDZ
CSB-BP321835EDZ
CSB-MP321835EDZ
CSB-EP321835EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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