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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Translation initiation factor IF-1 (infA) CSB-YP322645BRJ
CSB-EP322645BRJ
CSB-BP322645BRJ
CSB-MP322645BRJ
CSB-EP322645BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodosporidium toruloides Rhodotorucin-A peptides type 2 (RHA2) CSB-YP322646REB
CSB-EP322646REB
CSB-BP322646REB
CSB-MP322646REB
CSB-EP322646REB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bunyamwera virus RNA-directed RNA polymerase L (L), partial CSB-YP322647BNM
CSB-EP322647BNM
CSB-BP322647BNM
CSB-MP322647BNM
CSB-EP322647BNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trimeresurus gramineus Phospholipase A2 isozyme 1 (PLA1) CSB-YP322648TQK
CSB-EP322648TQK
CSB-BP322648TQK
CSB-MP322648TQK
CSB-EP322648TQK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein MAK11 (MAK11) CSB-YP322649SVG
CSB-EP322649SVG
CSB-BP322649SVG
CSB-MP322649SVG
CSB-EP322649SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Dual specificity protein phosphatase H1 (H1L) CSB-YP322650VAA
CSB-EP322650VAA
CSB-BP322650VAA
CSB-MP322650VAA
CSB-EP322650VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein I5 (I5L), partial CSB-YP322651VAA1
CSB-EP322651VAA1
CSB-BP322651VAA1
CSB-MP322651VAA1
CSB-EP322651VAA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus B1 kinase (OPG187) CSB-YP322652VAA
CSB-EP322652VAA
CSB-BP322652VAA
CSB-MP322652VAA
CSB-EP322652VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Cell surface-binding protein OPG105 (OPG105) CSB-YP322653VAA
CSB-BP322653VAA
CSB-MP322653VAA
CSB-EP322653VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 18.2 kDa protein (A ORF E) CSB-YP322654VAA
CSB-EP322654VAA
CSB-BP322654VAA
CSB-MP322654VAA
CSB-EP322654VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 7.9 kDa protein (A ORF J) CSB-YP322655VAA
CSB-EP322655VAA
CSB-BP322655VAA
CSB-MP322655VAA
CSB-EP322655VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 9.9 kDa protein (A ORF P) CSB-YP322656VAA
CSB-EP322656VAA
CSB-BP322656VAA
CSB-MP322656VAA
CSB-EP322656VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 8.8 kDa protein (A ORF U) CSB-YP322657VAA
CSB-EP322657VAA
CSB-BP322657VAA
CSB-MP322657VAA
CSB-EP322657VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 7.9 kDa protein (B ORF B) CSB-YP322658VAA
CSB-EP322658VAA
CSB-BP322658VAA
CSB-MP322658VAA
CSB-EP322658VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 9.2 kDa protein (D ORF A) CSB-YP322659VAA
CSB-EP322659VAA
CSB-BP322659VAA
CSB-MP322659VAA
CSB-EP322659VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 8.0 kDa protein (E ORF C) CSB-YP322660VAA
CSB-EP322660VAA
CSB-BP322660VAA
CSB-MP322660VAA
CSB-EP322660VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 8.3 kDa protein (F ORF B) CSB-YP322661VAA
CSB-EP322661VAA
CSB-BP322661VAA
CSB-MP322661VAA
CSB-EP322661VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 8.4 kDa protein (G ORF B) CSB-YP322662VAA
CSB-EP322662VAA
CSB-BP322662VAA
CSB-MP322662VAA
CSB-EP322662VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 9.3 kDa protein (I ORF A) CSB-YP322663VAA
CSB-EP322663VAA
CSB-BP322663VAA
CSB-MP322663VAA
CSB-EP322663VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Anthranilate phosphoribosyltransferase (trpD) CSB-YP322664FFZ
CSB-EP322664FFZ
CSB-BP322664FFZ
CSB-MP322664FFZ
CSB-EP322664FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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