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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Vaccinia virus Protein A40 (A40R), partial CSB-YP322726VAA
CSB-EP322726VAA
CSB-BP322726VAA
CSB-MP322726VAA
CSB-EP322726VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein A49 (A49R) CSB-YP322727VAA
CSB-EP322727VAA
CSB-BP322727VAA
CSB-MP322727VAA
CSB-EP322727VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein B17 (B17L) CSB-YP322728VAA
CSB-EP322728VAA
CSB-BP322728VAA
CSB-MP322728VAA
CSB-EP322728VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus RNA-binding protein OPG065 (OPG065) CSB-BP322729VAA
CSB-MP322729VAA
CSB-EP322729VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein K5 (K5L) CSB-YP322730VAA
CSB-EP322730VAA
CSB-BP322730VAA
CSB-MP322730VAA
CSB-EP322730VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein O1 (O1L), partial CSB-YP322731VAA1
CSB-EP322731VAA1
CSB-BP322731VAA1
CSB-MP322731VAA1
CSB-EP322731VAA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein C16/B22 (B22R) CSB-YP322732VAA
CSB-EP322732VAA
CSB-BP322732VAA
CSB-MP322732VAA
CSB-EP322732VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein C21/B27 (B27R) CSB-YP322733VAA
CSB-EP322733VAA
CSB-BP322733VAA
CSB-MP322733VAA
CSB-EP322733VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Putative A-type inclusion protein (A25L) CSB-YP322734VAA
CSB-EP322734VAA
CSB-BP322734VAA
CSB-MP322734VAA
CSB-EP322734VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 8.0 kDa protein (C ORF A) CSB-YP322735VAA
CSB-EP322735VAA
CSB-BP322735VAA
CSB-MP322735VAA
CSB-EP322735VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caprine arthritis encephalitis virus Probable Vpr-like protein (tat) CSB-YP322736CDF
CSB-EP322736CDF
CSB-BP322736CDF
CSB-MP322736CDF
CSB-EP322736CDF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Cu-Zn superoxide dismutase-like protein (A45R) CSB-YP322737VAA
CSB-EP322737VAA
CSB-BP322737VAA
CSB-MP322737VAA
CSB-EP322737VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African cassava mosaic virus Replication enhancer protein (AC3, AL3) CSB-YP322738ADW
CSB-EP322738ADW
CSB-BP322738ADW
CSB-MP322738ADW
CSB-EP322738ADW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African cassava mosaic virus Movement protein BC1 (BC1, BL1) CSB-YP322739ADX
CSB-EP322739ADX
CSB-BP322739ADX
CSB-MP322739ADX
CSB-EP322739ADX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maize streak virus genotype A Movement protein (V2), partial CSB-YP322740MBL1
CSB-EP322740MBL1
CSB-BP322740MBL1
CSB-MP322740MBL1
CSB-EP322740MBL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Phytochrome A (PHYA), partial CSB-YP322741EWE
CSB-EP322741EWE
CSB-BP322741EWE
CSB-MP322741EWE
CSB-EP322741EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase protein I (atpI), partial CSB-YP322742RMA1
CSB-EP322742RMA1
CSB-BP322742RMA1
CSB-MP322742RMA1
CSB-EP322742RMA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 10 kDa chaperonin (groS) CSB-YP322743MVH
CSB-EP322743MVH
CSB-BP322743MVH
CSB-MP322743MVH
CSB-EP322743MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Fe (3+) dicitrate transport system permease protein fecC, partial CSB-YP322744ENV
CSB-EP322744ENV
CSB-BP322744ENV
CSB-MP322744ENV
CSB-EP322744ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli DNA ligase (ligA) CSB-YP322745ENV
CSB-BP322745ENV
CSB-MP322745ENV
CSB-EP322745ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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