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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Enterobacteria phage T3 Gene 4.3 protein (4.3) CSB-YP322625EDY
CSB-EP322625EDY
CSB-BP322625EDY
CSB-MP322625EDY
CSB-EP322625EDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T3 Gene 5.7 protein (5.7) CSB-YP322626EDY
CSB-EP322626EDY
CSB-BP322626EDY
CSB-MP322626EDY
CSB-EP322626EDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T3 Capsid assembly protein (9) CSB-YP322627EDY
CSB-EP322627EDY
CSB-BP322627EDY
CSB-MP322627EDY
CSB-EP322627EDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T3 Uncharacterized gene 5.3 protein (5.3) CSB-YP322628EDY
CSB-EP322628EDY
CSB-BP322628EDY
CSB-MP322628EDY
CSB-EP322628EDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative uncharacterized protein CysX (cysX) CSB-YP322629ENV
CSB-EP322629ENV
CSB-BP322629ENV
CSB-MP322629ENV
CSB-EP322629ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum Cysteine protease inhibitor 1 CSB-YP322630FIG
CSB-EP322630FIG
CSB-BP322630FIG
CSB-MP322630FIG
CSB-EP322630FIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Guanine nucleotide-binding protein G (i) subunit alpha 65A CSB-YP322631DLU
CSB-EP322631DLU
CSB-BP322631DLU
CSB-MP322631DLU
CSB-EP322631DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actin, cytoplasmic CSB-YP322632EPN
CSB-EP322632EPN
CSB-BP322632EPN
CSB-MP322632EPN
CSB-EP322632EPN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative transposon Tn552 DNA-invertase bin3 (bin3) CSB-YP322633FKZ
CSB-EP322633FKZ
CSB-BP322633FKZ
CSB-MP322633FKZ
CSB-EP322633FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heloderma horridum horridum Exendin-3 CSB-YP322634HDE
CSB-EP322634HDE
CSB-BP322634HDE
CSB-MP322634HDE
CSB-EP322634HDE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Paralemmin-3 (palm3), partial CSB-YP322635XBE
CSB-EP322635XBE
CSB-BP322635XBE
CSB-MP322635XBE
CSB-EP322635XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein-tyrosine phosphatase rolB (rolB) CSB-YP322636AEW
CSB-EP322636AEW
CSB-BP322636AEW
CSB-MP322636AEW
CSB-EP322636AEW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Locusta migratoria Locustapyrokinin-1 CSB-YP322637LHY
CSB-EP322637LHY
CSB-BP322637LHY
CSB-MP322637LHY
CSB-EP322637LHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bothrops jararaca Zinc metalloproteinase bothrolysin CSB-YP322638BUW
CSB-EP322638BUW
CSB-BP322638BUW
CSB-MP322638BUW
CSB-EP322638BUW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phospholipase C (plc) CSB-YP322639DUD
CSB-EP322639DUD
CSB-BP322639DUD
CSB-MP322639DUD
CSB-EP322639DUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Myoxocephalus aenaeus Ice-structuring protein GS-5 CSB-YP322640MMF
CSB-EP322640MMF
CSB-BP322640MMF
CSB-MP322640MMF
CSB-EP322640MMF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP322641BRJ
CSB-EP322641BRJ
CSB-BP322641BRJ
CSB-MP322641BRJ
CSB-EP322641BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae G1/S-specific cyclin CLN1 (CLN1), partial CSB-YP322642SVG
CSB-EP322642SVG
CSB-BP322642SVG
CSB-MP322642SVG
CSB-EP322642SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anthranilate synthase component II (trpG) CSB-YP322643LER
CSB-EP322643LER
CSB-BP322643LER
CSB-MP322643LER
CSB-EP322643LER-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Photosystem I assembly protein Ycf4 (ycf4), partial CSB-YP322644HWQ1
CSB-EP322644HWQ1
CSB-BP322644HWQ1
CSB-MP322644HWQ1
CSB-EP322644HWQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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