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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Gibberella baccata Extracellular guanyl-specific ribonuclease Fl1 CSB-YP322849GBX
CSB-EP322849GBX
CSB-BP322849GBX
CSB-MP322849GBX
CSB-EP322849GBX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Liophis miliaris Hemoglobin subunit alpha-D (HBAD) CSB-YP322850LGM
CSB-EP322850LGM
CSB-BP322850LGM
CSB-MP322850LGM
CSB-EP322850LGM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Retrovirus-related Pol polyprotein from type-2 retrotransposable element R2DM (pol), partial CSB-YP322851DLU
CSB-EP322851DLU
CSB-BP322851DLU
CSB-MP322851DLU
CSB-EP322851DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Formate hydrogenlyase subunit 6 (hycF) CSB-YP322852ENV
CSB-EP322852ENV
CSB-BP322852ENV
CSB-MP322852ENV
CSB-EP322852ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clover yellow mosaic virus Coat protein CSB-YP322854CMQ
CSB-EP322854CMQ
CSB-BP322854CMQ
CSB-MP322854CMQ
CSB-EP322854CMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hantaan virus Envelopment polyprotein (GP), partial CSB-YP322855HCD1
CSB-EP322855HCD1
CSB-BP322855HCD1
CSB-MP322855HCD1
CSB-EP322855HCD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus RNA-directed RNA polymerase catalytic subunit (PB1), partial CSB-YP322856IMJ
CSB-EP322856IMJ
CSB-BP322856IMJ
CSB-MP322856IMJ
CSB-EP322856IMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus RNA-directed RNA polymerase catalytic subunit (PB1), partial CSB-YP322857IIN
CSB-EP322857IIN
CSB-BP322857IIN
CSB-MP322857IIN
CSB-EP322857IIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Elongation factor 3A (YEF3), partial CSB-YP322858SVG
CSB-EP322858SVG
CSB-BP322858SVG
CSB-MP322858SVG
CSB-EP322858SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative pectate lyase X (PEL X) CSB-YP322859EMX
CSB-EP322859EMX
CSB-BP322859EMX
CSB-MP322859EMX
CSB-EP322859EMX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tetracenomycin C polyketide putative beta-ketoacyl synthase 1 (tcmK) CSB-YP322860SOG
CSB-EP322860SOG
CSB-BP322860SOG
CSB-MP322860SOG
CSB-EP322860SOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein numb (numb), partial CSB-YP322861DLU
CSB-EP322861DLU
CSB-BP322861DLU
CSB-MP322861DLU
CSB-EP322861DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fremyella diplosiphon Phycobiliprotein ApcE (apcE), partial CSB-YP322862FCY
CSB-EP322862FCY
CSB-BP322862FCY
CSB-MP322862FCY
CSB-EP322862FCY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprinopsis cinerea Tryptophan synthase (TRP-1), partial CSB-YP322863DWP
CSB-EP322863DWP
CSB-BP322863DWP
CSB-MP322863DWP
CSB-EP322863DWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Tryptophan synthase beta chain (trpB) CSB-YP322864TNS
CSB-EP322864TNS
CSB-BP322864TNS
CSB-MP322864TNS
CSB-EP322864TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Glutamyl-tRNA reductase (hemA) CSB-YP322865BRJ
CSB-EP322865BRJ
CSB-BP322865BRJ
CSB-MP322865BRJ
CSB-EP322865BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 10 kDa chaperonin (groS) CSB-YP322866OCJ
CSB-EP322866OCJ
CSB-BP322866OCJ
CSB-MP322866OCJ
CSB-EP322866OCJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gracilaria tenuistipitata 50S ribosomal protein L16, chloroplastic (rpl16) CSB-YP322867GDC
CSB-EP322867GDC
CSB-BP322867GDC
CSB-MP322867GDC
CSB-EP322867GDC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schistosoma mansoni Taurocyamine kinase (Smp_194770), partial CSB-YP322868SXQ
CSB-EP322868SXQ
CSB-BP322868SXQ
CSB-MP322868SXQ
CSB-EP322868SXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Potato virus S Movement protein TGB2 (ORF3), partial CSB-YP322869PRB
CSB-EP322869PRB
CSB-BP322869PRB
CSB-MP322869PRB
CSB-EP322869PRB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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