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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Human immunodeficiency virus type 2 subtype B Protein Nef (nef) CSB-YP322809HMD
CSB-EP322809HMD
CSB-BP322809HMD
CSB-MP322809HMD
CSB-EP322809HMD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype B Protein Vpx (vpx) CSB-YP322810HMD
CSB-EP322810HMD
CSB-BP322810HMD
CSB-MP322810HMD
CSB-EP322810HMD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Homeobox protein Hox-C6a (hoxc6a) CSB-YP322811DIL
CSB-EP322811DIL
CSB-BP322811DIL
CSB-MP322811DIL
CSB-EP322811DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Strongylocentrotus purpuratus Histone H1-delta CSB-YP322812FOT
CSB-EP322812FOT
CSB-BP322812FOT
CSB-MP322812FOT
CSB-EP322812FOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Actin-binding protein (ABP1), partial CSB-YP322813SVG
CSB-EP322813SVG
CSB-BP322813SVG
CSB-MP322813SVG
CSB-EP322813SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spiroplasma virus SpV1-R8A2 B Putative capsid protein ORF9 (ORF9), partial CSB-YP322814SJZ
CSB-EP322814SJZ
CSB-BP322814SJZ
CSB-MP322814SJZ
CSB-EP322814SJZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fowlpox virus Protein L1 homolog (FPV128), partial CSB-YP322815FEY1
CSB-EP322815FEY1
CSB-BP322815FEY1
CSB-MP322815FEY1
CSB-EP322815FEY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human V (D)J recombination-activating protein 1, partial CSB-YP322816HU
CSB-EP322816HU
CSB-BP322816HU
CSB-MP322816HU
CSB-EP322816HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Nodulation protein W (nodW) CSB-YP322817BVW
CSB-EP322817BVW
CSB-BP322817BVW
CSB-MP322817BVW
CSB-EP322817BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Autographa californica nuclear polyhedrosis virus Protein p74 (P74), partial CSB-YP322818ARA
CSB-EP322818ARA
CSB-BP322818ARA
CSB-MP322818ARA
CSB-EP322818ARA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agelenopsis aperta Omega-agatoxin-Aa1b CSB-YP322819AER
CSB-EP322819AER
CSB-BP322819AER
CSB-MP322819AER
CSB-EP322819AER-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig SLA class II histocompatibility antigen, DQ haplotype C beta chain, partial CSB-YP322820PI
CSB-EP322820PI
CSB-BP322820PI
CSB-MP322820PI
CSB-EP322820PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui Elongation factor 1-alpha (tuf) CSB-YP322821HTJ
CSB-EP322821HTJ
CSB-BP322821HTJ
CSB-MP322821HTJ
CSB-EP322821HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays DNA-directed RNA polymerase subunit beta' (rpoC2), partial CSB-YP322822ZAX
CSB-EP322822ZAX
CSB-BP322822ZAX
CSB-MP322822ZAX
CSB-EP322822ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Magnetospirillum magnetotacticum Protein RecA (recA) CSB-YP322823MPN
CSB-EP322823MPN
CSB-BP322823MPN
CSB-MP322823MPN
CSB-EP322823MPN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Strongylocentrotus purpuratus Speract receptor, partial CSB-YP322824FOT
CSB-EP322824FOT
CSB-BP322824FOT
CSB-MP322824FOT
CSB-EP322824FOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mumps virus Phosphoprotein (P/V) CSB-YP322825MJF
CSB-EP322825MJF
CSB-BP322825MJF
CSB-MP322825MJF
CSB-EP322825MJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Feline immunodeficiency virus Virion infectivity factor (vif) CSB-YP322826FAK
CSB-EP322826FAK
CSB-BP322826FAK
CSB-MP322826FAK
CSB-EP322826FAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesembryanthemum crystallinum Phosphoenolpyruvate carboxylase 2 (PPC2), partial CSB-YP322827MRC
CSB-EP322827MRC
CSB-BP322827MRC
CSB-MP322827MRC
CSB-EP322827MRC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Repressor protein CI (CI) CSB-YP322828BDJ
CSB-EP322828BDJ
CSB-BP322828BDJ
CSB-MP322828BDJ
CSB-EP322828BDJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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