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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Romalea microptera RO-1 CSB-YP322993RFF
CSB-EP322993RFF
CSB-BP322993RFF
CSB-MP322993RFF
CSB-EP322993RFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Catalase isozyme 3 (CAT3) CSB-YP322994ZAX
CSB-EP322994ZAX
CSB-BP322994ZAX
CSB-MP322994ZAX
CSB-EP322994ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized 26.4 kDa protein CSB-YP322995ENL
CSB-EP322995ENL
CSB-BP322995ENL
CSB-MP322995ENL
CSB-EP322995ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum cAMP-regulated D2 protein (D2), partial CSB-YP322996DKK
CSB-EP322996DKK
CSB-BP322996DKK
CSB-MP322996DKK
CSB-EP322996DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica 13 kDa major membrane protein (FTL_0420) CSB-YP322997FCO
CSB-EP322997FCO
CSB-BP322997FCO
CSB-MP322997FCO
CSB-EP322997FCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Phosphoglucomutase (pgcA), partial CSB-YP322998BRJ
CSB-EP322998BRJ
CSB-BP322998BRJ
CSB-MP322998BRJ
CSB-EP322998BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Glucose dehydrogenase [acceptor] (Gld), partial CSB-YP322999DLU
CSB-EP322999DLU
CSB-BP322999DLU
CSB-MP322999DLU
CSB-EP322999DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces coelicolor RNA polymerase principal sigma factor hrdB (hrdB), partial CSB-YP323000FOB
CSB-EP323000FOB
CSB-BP323000FOB
CSB-MP323000FOB
CSB-EP323000FOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uptake hydrogenase large subunit (hupL), partial CSB-YP323001DPU
CSB-EP323001DPU
CSB-BP323001DPU
CSB-MP323001DPU
CSB-EP323001DPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-isopropylmalate dehydratase (leu1), partial CSB-YP323004PIL
CSB-EP323004PIL
CSB-BP323004PIL
CSB-MP323004PIL
CSB-EP323004PIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracentrotus lividus Tubulin alpha-1 chain CSB-YP323005ERM
CSB-EP323005ERM
CSB-BP323005ERM
CSB-MP323005ERM
CSB-EP323005ERM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii Photosystem II reaction center protein K (psbK) CSB-YP323006DSJ
CSB-EP323006DSJ
CSB-BP323006DSJ
CSB-MP323006DSJ
CSB-EP323006DSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sheep Chymosin (CYM) CSB-YP323007SH
CSB-EP323007SH
CSB-BP323007SH
CSB-MP323007SH
CSB-EP323007SH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Isopenicillin N synthase (pcbC) CSB-YP323008SOP
CSB-EP323008SOP
CSB-BP323008SOP
CSB-MP323008SOP
CSB-EP323008SOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter aerogenes Urease accessory protein UreE (ureE) CSB-YP323009EKN
CSB-EP323009EKN
CSB-BP323009EKN
CSB-MP323009EKN
CSB-EP323009EKN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Acetylornithine/succinyldiaminopimelate aminotransferase (argD) CSB-YP323010ENV
CSB-EP323010ENV
CSB-BP323010ENV
CSB-MP323010ENV
CSB-EP323010ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equine herpesvirus 4 Envelope glycoprotein E (gE), partial CSB-YP323011EFR1
CSB-EP323011EFR1
CSB-BP323011EFR1
CSB-MP323011EFR1
CSB-EP323011EFR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized 27.6 kDa protein CSB-YP323012ENL
CSB-EP323012ENL
CSB-BP323012ENL
CSB-MP323012ENL
CSB-EP323012ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 5.3 kDa protein CSB-YP323013VAI
CSB-EP323013VAI
CSB-BP323013VAI
CSB-MP323013VAI
CSB-EP323013VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yjjA (yjjA) CSB-YP323014ENV
CSB-EP323014ENV
CSB-BP323014ENV
CSB-MP323014ENV
CSB-EP323014ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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