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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Insertion sequence IS21 putative ATP-binding protein (istB) CSB-YP324439EZW
CSB-EP324439EZW
CSB-BP324439EZW
CSB-MP324439EZW
CSB-EP324439EZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Serine proteinase inhibitor 1 (SPI-1) CSB-YP324440VAI
CSB-EP324440VAI
CSB-BP324440VAI
CSB-MP324440VAI
CSB-EP324440VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein traH (traH) CSB-YP324441ENV
CSB-EP324441ENV
CSB-BP324441ENV
CSB-MP324441ENV
CSB-EP324441ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Carbon starvation protein A (cstA), partial CSB-YP324442ENV
CSB-EP324442ENV
CSB-BP324442ENV
CSB-MP324442ENV
CSB-EP324442ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Narcissus mosaic virus Movement protein TGBp3 (ORF4), partial CSB-YP324443NAR
CSB-EP324443NAR
CSB-BP324443NAR
CSB-MP324443NAR
CSB-EP324443NAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Metallothionein-1 (MT-I) CSB-YP324444CZI
CSB-EP324444CZI
CSB-BP324444CZI
CSB-MP324444CZI
CSB-EP324444CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Locusta migratoria LIRP CSB-YP324445LHY
CSB-EP324445LHY
CSB-BP324445LHY
CSB-MP324445LHY
CSB-EP324445LHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cucumber necrosis virus Capsid protein (ORF2) CSB-YP324446CUF
CSB-EP324446CUF
CSB-BP324446CUF
CSB-MP324446CUF
CSB-EP324446CUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nucleoprotein (N) CSB-YP324447DHK
CSB-EP324447DHK
CSB-BP324447DHK
CSB-MP324447DHK
CSB-EP324447DHK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabies virus Phosphoprotein (P) CSB-YP324448RAI
CSB-EP324448RAI
CSB-BP324448RAI
CSB-MP324448RAI
CSB-EP324448RAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Strongylocentrotus purpuratus Fibropellin-2 (EGF2) CSB-YP324449FOT
CSB-EP324449FOT
CSB-BP324449FOT
CSB-MP324449FOT
CSB-EP324449FOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesobuthus martensii Neurotoxin BmK-M3 CSB-YP324450MRE
CSB-EP324450MRE
CSB-BP324450MRE
CSB-MP324450MRE
CSB-EP324450MRE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage N4 Uncharacterized protein Gp2 CSB-YP324451EDB
CSB-EP324451EDB
CSB-BP324451EDB
CSB-MP324451EDB
CSB-EP324451EDB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromosomal protein MC1b CSB-YP324452MFH
CSB-EP324452MFH
CSB-BP324452MFH
CSB-MP324452MFH
CSB-EP324452MFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zymomonas mobilis subsp. mobilis 20.9 kDa protein (Zmob_1875) CSB-YP324453ZBN
CSB-EP324453ZBN
CSB-BP324453ZBN
CSB-MP324453ZBN
CSB-EP324453ZBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Transcriptional regulatory protein AlgQ (algQ) CSB-YP324454EZX
CSB-EP324454EZX
CSB-BP324454EZX
CSB-MP324454EZX
CSB-EP324454EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus Uptake hydrogenase large subunit (hupB), partial CSB-YP324455RLC
CSB-EP324455RLC
CSB-BP324455RLC
CSB-MP324455RLC
CSB-EP324455RLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris PIII-type proteinase (prtP), partial CSB-YP324456LBE
CSB-EP324456LBE
CSB-BP324456LBE
CSB-MP324456LBE
CSB-EP324456LBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein B4 (b4) CSB-YP324457XBE
CSB-EP324457XBE
CSB-BP324457XBE
CSB-MP324457XBE
CSB-EP324457XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized 65 kDa protein in hyaluronidase region, partial CSB-YP324458SMS
CSB-EP324458SMS
CSB-BP324458SMS
CSB-MP324458SMS
CSB-EP324458SMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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