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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli Colicin-E5 (col), partial CSB-EP323819ENL1
CSB-BP323819ENL1
CSB-MP323819ENL1
CSB-EP323819ENL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Uncharacterized protein EF_A0048 (EF_A0048) CSB-YP323820ELW
CSB-EP323820ELW
CSB-BP323820ELW
CSB-MP323820ELW
CSB-EP323820ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Replication protein (rep) CSB-YP323821CMB
CSB-EP323821CMB
CSB-BP323821CMB
CSB-MP323821CMB
CSB-EP323821CMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Abortive infection protein (abi) CSB-YP323822ENL
CSB-EP323822ENL
CSB-BP323822ENL
CSB-MP323822ENL
CSB-EP323822ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein traQ (traQ), partial CSB-YP323823ENV1
CSB-EP323823ENV1
CSB-BP323823ENV1
CSB-MP323823ENV1
CSB-EP323823ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype A Protein Rev (rev) CSB-YP323824HLX
CSB-EP323824HLX
CSB-BP323824HLX
CSB-MP323824HLX
CSB-EP323824HLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype A Protein Vpr (vpr) CSB-YP323825HLX
CSB-EP323825HLX
CSB-BP323825HLX
CSB-MP323825HLX
CSB-EP323825HLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Highly immunogenic outer capsid protein (hoc) CSB-YP323826EDZ
CSB-EP323826EDZ
CSB-BP323826EDZ
CSB-MP323826EDZ
CSB-EP323826EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribulose bisphosphate carboxylase small chain (rbcS) CSB-YP323827DZX
CSB-EP323827DZX
CSB-BP323827DZX
CSB-MP323827DZX
CSB-EP323827DZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Nucleoprotein (NP) CSB-YP323828IBG
CSB-EP323828IBG
CSB-BP323828IBG
CSB-MP323828IBG
CSB-EP323828IBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Catalase isozyme 1 (CAT1) CSB-YP323829ZAX
CSB-EP323829ZAX
CSB-BP323829ZAX
CSB-MP323829ZAX
CSB-EP323829ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ig kappa chain V-III region HAH CSB-YP323830HU
CSB-EP323830HU
CSB-BP323830HU
CSB-MP323830HU
CSB-EP323830HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Glycerol kinase (glpK) CSB-YP323832BRJ
CSB-EP323832BRJ
CSB-BP323832BRJ
CSB-MP323832BRJ
CSB-EP323832BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Frog virus 3 Early 31 kDa protein CSB-YP323833FDG
CSB-EP323833FDG
CSB-BP323833FDG
CSB-MP323833FDG
CSB-EP323833FDG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Periplasmic [NiFe] hydrogenase small subunit (hydA) CSB-YP323834DDD
CSB-EP323834DDD
CSB-BP323834DDD
CSB-MP323834DDD
CSB-EP323834DDD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Major outer membrane protein P.IA (porA) CSB-YP323835NGI
CSB-EP323835NGI
CSB-BP323835NGI
CSB-MP323835NGI
CSB-EP323835NGI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces coelicolor RNA polymerase principal sigma factor hrdD (hrdD) CSB-YP323836FOB
CSB-EP323836FOB
CSB-BP323836FOB
CSB-MP323836FOB
CSB-EP323836FOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Threonine--tRNA ligase 1 (thrS), partial CSB-YP323837BRJ
CSB-EP323837BRJ
CSB-BP323837BRJ
CSB-MP323837BRJ
CSB-EP323837BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis Homeobox protein rough (ro) CSB-YP323838DMP
CSB-EP323838DMP
CSB-BP323838DMP
CSB-MP323838DMP
CSB-EP323838DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trypanosoma cruzi Sialidase 85-1.3 (SA85-1.3), partial CSB-YP323839TQY
CSB-EP323839TQY
CSB-BP323839TQY
CSB-MP323839TQY
CSB-EP323839TQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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