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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Nodulation protein G (nodG) CSB-YP322952DPS
CSB-EP322952DPS
CSB-BP322952DPS
CSB-MP322952DPS
CSB-EP322952DPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Riboflavin biosynthesis protein RibD (ribD) CSB-YP322953BRJ
CSB-EP322953BRJ
CSB-BP322953BRJ
CSB-MP322953BRJ
CSB-EP322953BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avian infectious bronchitis virus Spike glycoprotein (S), partial CSB-YP322954ARQ
CSB-BP322954ARQ
CSB-MP322954ARQ
CSB-EP322954ARQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trypsin inhibitor 1 CSB-YP322955MHS
CSB-EP322955MHS
CSB-BP322955MHS
CSB-MP322955MHS
CSB-EP322955MHS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus sp. Cyclomaltodextrin glucanotransferase (cgt), partial CSB-YP322956BBX
CSB-EP322956BBX
CSB-BP322956BBX
CSB-MP322956BBX
CSB-EP322956BBX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydra vulgaris Tyrosine-protein kinase STK (STK), partial CSB-YP322957HXN
CSB-EP322957HXN
CSB-BP322957HXN
CSB-MP322957HXN
CSB-EP322957HXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leiurus quinquestriatus hebraeus Alpha-insect toxin LqhaIT CSB-YP322958LDS
CSB-BP322958LDS
CSB-MP322958LDS
CSB-EP322958LDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloferax volcanii Histidinol-phosphate aminotransferase (hisC) CSB-YP322959HTU
CSB-EP322959HTU
CSB-BP322959HTU
CSB-MP322959HTU
CSB-EP322959HTU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Elongation factor Tu, chloroplastic (TUFA) CSB-YP322960DOA
CSB-EP322960DOA
CSB-BP322960DOA
CSB-MP322960DOA
CSB-EP322960DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype A Protein Nef (nef) CSB-YP322961HLW
CSB-EP322961HLW
CSB-BP322961HLW
CSB-MP322961HLW
CSB-EP322961HLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype A Virion infectivity factor (vif) CSB-YP322962HLW
CSB-EP322962HLW
CSB-BP322962HLW
CSB-MP322962HLW
CSB-EP322962HLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Genome polyprotein, partial CSB-YP322963BFU
CSB-EP322963BFU
CSB-BP322963BFU
CSB-MP322963BFU
CSB-EP322963BFU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Organ-specific protein P4 CSB-YP322964EWE
CSB-EP322964EWE
CSB-BP322964EWE
CSB-MP322964EWE
CSB-EP322964EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Potato virus X RNA replication protein, partial CSB-YP322965PRF
CSB-EP322965PRF
CSB-BP322965PRF
CSB-MP322965PRF
CSB-EP322965PRF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Protein virB5 (virB5) CSB-YP322966AYS
CSB-EP322966AYS
CSB-BP322966AYS
CSB-MP322966AYS
CSB-EP322966AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leishmania donovani Heat shock 70 kDa protein (HSP70), partial CSB-YP322967LOE
CSB-EP322967LOE
CSB-BP322967LOE
CSB-MP322967LOE
CSB-EP322967LOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable 4-coumarate--CoA ligase 1 (4CL1), partial CSB-YP322968OFG
CSB-EP322968OFG
CSB-BP322968OFG
CSB-MP322968OFG
CSB-EP322968OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Matthiola incana Chalcone synthase (CHS) CSB-YP322969MQK
CSB-EP322969MQK
CSB-BP322969MQK
CSB-MP322969MQK
CSB-EP322969MQK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus Fimbrial protein (fimA) CSB-YP322970DDR
CSB-EP322970DDR
CSB-BP322970DDR
CSB-MP322970DDR
CSB-EP322970DDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60S ribosomal protein L44 (RPL44) CSB-YP322972TQV
CSB-EP322972TQV
CSB-BP322972TQV
CSB-MP322972TQV
CSB-EP322972TQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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