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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Drosophila melanogaster Fasciclin-3 (Fas3), partial CSB-YP323612DLU
CSB-EP323612DLU
CSB-BP323612DLU
CSB-MP323612DLU
CSB-EP323612DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Cytosol non-specific dipeptidase (pepD) CSB-YP323613ENV
CSB-EP323613ENV
CSB-BP323613ENV
CSB-MP323613ENV
CSB-EP323613ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus mykiss Dynein heavy chain, partial CSB-YP323614OEI
CSB-EP323614OEI
CSB-BP323614OEI
CSB-MP323614OEI
CSB-EP323614OEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hemolysin transporter protein shlB (shlB), partial CSB-YP323615SYN
CSB-EP323615SYN
CSB-BP323615SYN
CSB-MP323615SYN
CSB-EP323615SYN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Embryonic polarity protein dorsal (dl), partial CSB-YP323616DLU
CSB-EP323616DLU
CSB-BP323616DLU
CSB-MP323616DLU
CSB-EP323616DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium sp. 2,5-diketo-D-gluconic acid reductase B (dkgB) CSB-YP323617CQQ
CSB-EP323617CQQ
CSB-BP323617CQQ
CSB-MP323617CQQ
CSB-EP323617CQQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus elongatus Iron stress-induced chlorophyll-binding protein (isiA), partial CSB-YP323618FPY1
CSB-EP323618FPY1
CSB-BP323618FPY1
CSB-MP323618FPY1
CSB-EP323618FPY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces coelicolor Glycerol operon regulatory protein (gylR) CSB-YP323619FOB
CSB-EP323619FOB
CSB-BP323619FOB
CSB-MP323619FOB
CSB-EP323619FOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Phosrestin-2 (Arr1) CSB-YP323620DLU
CSB-EP323620DLU
CSB-BP323620DLU
CSB-MP323620DLU
CSB-EP323620DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein rolB (TR) CSB-YP323621AEW
CSB-EP323621AEW
CSB-BP323621AEW
CSB-MP323621AEW
CSB-EP323621AEW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant White clover mosaic virus Movement protein TGBp3 (ORF4), partial CSB-YP323622WAL
CSB-EP323622WAL
CSB-BP323622WAL
CSB-MP323622WAL
CSB-EP323622WAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage I2-2 Tail virion protein G7P (VII), partial CSB-YP323623ECP
CSB-EP323623ECP
CSB-BP323623ECP
CSB-MP323623ECP
CSB-EP323623ECP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human coronavirus 229E Membrane protein (M), partial CSB-YP323624HIT1
CSB-BP323624HIT1
CSB-MP323624HIT1
CSB-EP323624HIT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 12S seed storage protein CRU4 (CRU4) CSB-YP323625DOA
CSB-EP323625DOA
CSB-BP323625DOA
CSB-MP323625DOA
CSB-EP323625DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum Patatin-B2 (PATB2) CSB-YP323626FIG
CSB-EP323626FIG
CSB-BP323626FIG
CSB-MP323626FIG
CSB-EP323626FIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Outer membrane usher protein CS3-2, partial CSB-YP323627ENL
CSB-EP323627ENL
CSB-BP323627ENL
CSB-MP323627ENL
CSB-EP323627ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Giardia intestinalis Giardin subunit beta CSB-YP323628GFW
CSB-EP323628GFW
CSB-BP323628GFW
CSB-MP323628GFW
CSB-EP323628GFW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorosome envelope protein B (csmB) CSB-YP323629PEK
CSB-EP323629PEK
CSB-BP323629PEK
CSB-MP323629PEK
CSB-EP323629PEK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Acyl carrier protein 3, chloroplastic (ACL1.3) CSB-YP323630HWQ
CSB-EP323630HWQ
CSB-BP323630HWQ
CSB-MP323630HWQ
CSB-EP323630HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas sp. Acylase ACY 1 (acyI), partial CSB-YP323631PVW
CSB-EP323631PVW
CSB-BP323631PVW
CSB-MP323631PVW
CSB-EP323631PVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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