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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Rhizobium meliloti Nitrogen fixation protein fixG (fixG), partial CSB-YP323015RKU1
CSB-EP323015RKU1
CSB-BP323015RKU1
CSB-MP323015RKU1
CSB-EP323015RKU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Phosphoadenosine phosphosulfate reductase (MET16) CSB-YP323016SVG
CSB-EP323016SVG
CSB-BP323016SVG
CSB-MP323016SVG
CSB-EP323016SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae ER lumen protein retaining receptor (ERD2), partial CSB-YP323017SVG1
CSB-EP323017SVG1
CSB-BP323017SVG1
CSB-MP323017SVG1
CSB-EP323017SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Endo-1,4-beta-xylanase A (xynA) CSB-YP323018BRJ
CSB-EP323018BRJ
CSB-BP323018BRJ
CSB-MP323018BRJ
CSB-EP323018BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Murine coronavirus Nucleoprotein (N) CSB-YP323019MKB
CSB-EP323019MKB
CSB-BP323019MKB
CSB-MP323019MKB
CSB-EP323019MKB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae SWI/SNF chromatin-remodeling complex subunit SNF5 (SNF5), partial CSB-YP323020SVG
CSB-EP323020SVG
CSB-BP323020SVG
CSB-MP323020SVG
CSB-EP323020SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein pecanex (pcx), partial CSB-YP323021DLU
CSB-EP323021DLU
CSB-BP323021DLU
CSB-MP323021DLU
CSB-EP323021DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc sp. Protein HesB (hesB) CSB-YP323022NHR
CSB-EP323022NHR
CSB-BP323022NHR
CSB-MP323022NHR
CSB-EP323022NHR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ig heavy chain V region 7-39 CSB-YP323023MO
CSB-EP323023MO
CSB-BP323023MO
CSB-MP323023MO
CSB-EP323023MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meleagrid herpesvirus 1 Envelope protein UL45 homolog, partial CSB-YP323024MDM1
CSB-EP323024MDM1
CSB-BP323024MDM1
CSB-MP323024MDM1
CSB-EP323024MDM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lymphocytic choriomeningitis virus RING finger protein Z (Z) CSB-YP323025LKW
CSB-EP323025LKW
CSB-BP323025LKW
CSB-MP323025LKW
CSB-EP323025LKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deacetoxycephalosporin C synthase (cefE) CSB-YP323026FOA
CSB-EP323026FOA
CSB-BP323026FOA
CSB-MP323026FOA
CSB-EP323026FOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus B Non-structural protein 5 CSB-YP323028RHE
CSB-EP323028RHE
CSB-BP323028RHE
CSB-MP323028RHE
CSB-EP323028RHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Small cysteine-rich outer membrane protein omcA, serovars L1/L2 (omcA) CSB-YP323029DSA
CSB-EP323029DSA
CSB-BP323029DSA
CSB-MP323029DSA
CSB-EP323029DSA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Protein virD3 (virD3), partial CSB-YP323030AYS
CSB-EP323030AYS
CSB-BP323030AYS
CSB-MP323030AYS
CSB-EP323030AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Artemia sp. Actin, clone 403 CSB-YP323031AOF
CSB-EP323031AOF
CSB-BP323031AOF
CSB-MP323031AOF
CSB-EP323031AOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Measles virus Matrix protein (M) CSB-YP323032MCT
CSB-EP323032MCT
CSB-BP323032MCT
CSB-MP323032MCT
CSB-EP323032MCT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Low-affinity glucose transporter (RAG1), partial CSB-YP323033KBK
CSB-EP323033KBK
CSB-BP323033KBK
CSB-MP323033KBK
CSB-EP323033KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uptake hydrogenase small subunit (hupA) CSB-YP323034RKR
CSB-EP323034RKR
CSB-BP323034RKR
CSB-MP323034RKR
CSB-EP323034RKR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vigna unguiculata Defensin-like protein CSB-YP323035VFM
CSB-EP323035VFM
CSB-BP323035VFM
CSB-MP323035VFM
CSB-EP323035VFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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