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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Vaccinia virus Virion membrane protein A21 (A21L), partial CSB-YP323306VAA
CSB-EP323306VAA
CSB-BP323306VAA
CSB-MP323306VAA
CSB-EP323306VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein B3 (B3R) CSB-YP323307VAA
CSB-EP323307VAA
CSB-BP323307VAA
CSB-MP323307VAA
CSB-EP323307VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Core protein D3 (D3R) CSB-YP323308VAA
CSB-EP323308VAA
CSB-BP323308VAA
CSB-MP323308VAA
CSB-EP323308VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus mRNA-decapping protein D10 (D10R) CSB-YP323309VAA
CSB-EP323309VAA
CSB-BP323309VAA
CSB-MP323309VAA
CSB-EP323309VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein F14 (F14L) CSB-YP323310VAA
CSB-EP323310VAA
CSB-BP323310VAA
CSB-MP323310VAA
CSB-EP323310VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Assembly protein G7 (G7L) CSB-YP323311VAA
CSB-EP323311VAA
CSB-BP323311VAA
CSB-MP323311VAA
CSB-EP323311VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus DNA-directed RNA polymerase 18 kDa subunit (RPO18) CSB-YP323312VAA
CSB-EP323312VAA
CSB-BP323312VAA
CSB-MP323312VAA
CSB-EP323312VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein C5 (C5L) CSB-YP323313VAA
CSB-EP323313VAA
CSB-BP323313VAA
CSB-MP323313VAA
CSB-EP323313VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein C13 (C13L) CSB-YP323314VAA
CSB-EP323314VAA
CSB-BP323314VAA
CSB-MP323314VAA
CSB-EP323314VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein E6 (E6R), partial CSB-YP323315VAA
CSB-EP323315VAA
CSB-BP323315VAA
CSB-MP323315VAA
CSB-EP323315VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein F12 (F12L), partial CSB-YP323316VAA
CSB-EP323316VAA
CSB-BP323316VAA
CSB-MP323316VAA
CSB-EP323316VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein A38 (A38L), partial CSB-YP323317VAA1
CSB-EP323317VAA1
CSB-BP323317VAA1
CSB-MP323317VAA1
CSB-EP323317VAA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein A51 (A51R) CSB-YP323318VAA
CSB-EP323318VAA
CSB-BP323318VAA
CSB-MP323318VAA
CSB-EP323318VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Kelch repeat and BTB domain-containing protein 1 (KBTB1), partial CSB-YP323319VAA
CSB-EP323319VAA
CSB-BP323319VAA
CSB-MP323319VAA
CSB-EP323319VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Surface antigen S (B19R) CSB-YP323320VAA
CSB-EP323320VAA
CSB-BP323320VAA
CSB-MP323320VAA
CSB-EP323320VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus DNA-directed RNA polymerase 30 kDa polypeptide (RPO30) CSB-YP323321VAA
CSB-EP323321VAA
CSB-BP323321VAA
CSB-MP323321VAA
CSB-EP323321VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein M2 (M2L) CSB-YP323322VAA
CSB-EP323322VAA
CSB-BP323322VAA
CSB-MP323322VAA
CSB-EP323322VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein C15/B21 (B21R) CSB-YP323323VAA
CSB-BP323323VAA
CSB-MP323323VAA
CSB-EP323323VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Truncated CrmB protein (B28R) CSB-YP323324VAA
CSB-EP323324VAA
CSB-BP323324VAA
CSB-MP323324VAA
CSB-EP323324VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter thermautotrophicus Methyl-coenzyme M reductase II subunit beta (mrtB) CSB-YP323325MSR
CSB-EP323325MSR
CSB-BP323325MSR
CSB-MP323325MSR
CSB-EP323325MSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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