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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vesicular stomatitis New Jersey virus RNA-directed RNA polymerase L (L), partial CSB-YP323428VBM
CSB-EP323428VBM
CSB-BP323428VBM
CSB-MP323428VBM
CSB-EP323428VBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium falciparum Octapeptide-repeat antigen, partial CSB-YP323429PMC
CSB-EP323429PMC
CSB-BP323429PMC
CSB-MP323429PMC
CSB-EP323429PMC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gibberella baccata Extracellular guanyl-specific ribonuclease Fl2 CSB-YP323430GBX
CSB-EP323430GBX
CSB-BP323430GBX
CSB-MP323430GBX
CSB-EP323430GBX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Putative 115 kDa protein in type-1 retrotransposable element R1DM, partial CSB-YP323431DLU
CSB-EP323431DLU
CSB-BP323431DLU
CSB-MP323431DLU
CSB-EP323431DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Pyruvate dehydrogenase complex protein X component, mitochondrial (PDX1) CSB-YP323432SVG
CSB-EP323432SVG
CSB-BP323432SVG
CSB-MP323432SVG
CSB-EP323432SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Telomere-binding protein subunit beta (MAC-41A) CSB-YP323433OGC
CSB-EP323433OGC
CSB-BP323433OGC
CSB-MP323433OGC
CSB-EP323433OGC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Pyruvate decarboxylase isozyme 2 (PDC5), partial CSB-YP323434SVG
CSB-EP323434SVG
CSB-BP323434SVG
CSB-MP323434SVG
CSB-EP323434SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein pndA (pndA), partial CSB-YP323435ENL
CSB-EP323435ENL
CSB-BP323435ENL
CSB-MP323435ENL
CSB-EP323435ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Intermediate capsid protein VP6 CSB-YP323436RNS
CSB-EP323436RNS
CSB-BP323436RNS
CSB-MP323436RNS
CSB-EP323436RNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bunyamwera virus Nucleoprotein (N) CSB-YP323437BNM
CSB-EP323437BNM
CSB-BP323437BNM
CSB-MP323437BNM
CSB-EP323437BNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus RNA-directed RNA polymerase catalytic subunit (PB1), partial CSB-YP323438IMG
CSB-EP323438IMG
CSB-BP323438IMG
CSB-MP323438IMG
CSB-EP323438IMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus RNA-directed RNA polymerase catalytic subunit (PB1), partial CSB-YP323439IGK
CSB-EP323439IGK
CSB-BP323439IGK
CSB-MP323439IGK
CSB-EP323439IGK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella haemolytica Leukotoxin-activating lysine-acyltransferase lktC serotype A1 (lktC) CSB-YP323440ESE
CSB-EP323440ESE
CSB-BP323440ESE
CSB-MP323440ESE
CSB-EP323440ESE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Granaticin polyketide putative beta-ketoacyl synthase 1 (gra-orf1) CSB-YP323441SQH
CSB-EP323441SQH
CSB-BP323441SQH
CSB-MP323441SQH
CSB-EP323441SQH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis subsp. spizizenii Xylose repressor (xylR) CSB-YP323442BRM
CSB-EP323442BRM
CSB-BP323442BRM
CSB-MP323442BRM
CSB-EP323442BRM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila abortus Major outer membrane porin (ompA) CSB-YP323443CJA
CSB-EP323443CJA
CSB-BP323443CJA
CSB-MP323443CJA
CSB-EP323443CJA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Ubiquitin-conjugating enzyme E2-23 kDa (UBC4) CSB-YP323444TQN
CSB-EP323444TQN
CSB-BP323444TQN
CSB-MP323444TQN
CSB-EP323444TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Papaya mosaic potexvirus Uncharacterized 14.7 kDa protein in coat protein coding strand CSB-YP323445PBN
CSB-EP323445PBN
CSB-BP323445PBN
CSB-MP323445PBN
CSB-EP323445PBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Non-structural protein NS-S (N) CSB-YP323446MBE
CSB-EP323446MBE
CSB-BP323446MBE
CSB-MP323446MBE
CSB-EP323446MBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Tyrosine-protein phosphatase 69D (Ptp69D), partial CSB-YP323447DLU
CSB-EP323447DLU
CSB-BP323447DLU
CSB-MP323447DLU
CSB-EP323447DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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