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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Vaccinia virus Interleukin-1-binding protein (B16R) CSB-YP323326VAA
CSB-EP323326VAA
CSB-BP323326VAA
CSB-MP323326VAA
CSB-EP323326VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 7.9 kDa protein (C ORF D) CSB-YP323327VAA
CSB-EP323327VAA
CSB-BP323327VAA
CSB-MP323327VAA
CSB-EP323327VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serine-rich 25 kDa antigen protein, partial CSB-YP323328EKM1
CSB-EP323328EKM1
CSB-BP323328EKM1
CSB-MP323328EKM1
CSB-EP323328EKM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African cassava mosaic virus Protein AV2 CSB-YP323329ADX
CSB-EP323329ADX
CSB-BP323329ADX
CSB-MP323329ADX
CSB-EP323329ADX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maize streak virus genotype A Replication-associated protein (C1/C2) CSB-YP323330MBK
CSB-EP323330MBK
CSB-BP323330MBK
CSB-MP323330MBK
CSB-EP323330MBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maize streak virus genotype A Replication-associated protein A (C1) CSB-YP323331MBL
CSB-EP323331MBL
CSB-BP323331MBL
CSB-MP323331MBL
CSB-EP323331MBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 5-methylcytosine-specific restriction enzyme B (mcrB) CSB-YP323332ENV
CSB-EP323332ENV
CSB-BP323332ENV
CSB-MP323332ENV
CSB-EP323332ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized transcriptional regulator in ATPase CF (0) region CSB-YP323333RMA
CSB-EP323333RMA
CSB-BP323333RMA
CSB-MP323333RMA
CSB-EP323333RMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transposase for insertion sequence element IS21 (istA) CSB-YP323334EZW
CSB-EP323334EZW
CSB-BP323334EZW
CSB-MP323334EZW
CSB-EP323334EZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Xaa-Pro aminopeptidase (pepP) CSB-YP323335ENV
CSB-EP323335ENV
CSB-BP323335ENV
CSB-MP323335ENV
CSB-EP323335ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thiobacillus ferrooxidans Nitrogenase molybdenum-iron protein beta chain (nifK), partial CSB-YP323336TOA
CSB-EP323336TOA
CSB-BP323336TOA
CSB-MP323336TOA
CSB-EP323336TOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glucitol operon activator protein (gutM) CSB-YP323337ENV
CSB-EP323337ENV
CSB-BP323337ENV
CSB-MP323337ENV
CSB-EP323337ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Narcissus mosaic virus Movement protein TGB2 (ORF3), partial CSB-YP323338NAR1
CSB-EP323338NAR1
CSB-BP323338NAR1
CSB-MP323338NAR1
CSB-EP323338NAR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus B serotype 7 Early E3B 14.9 kDa protein, partial CSB-YP323339HII1
CSB-EP323339HII1
CSB-BP323339HII1
CSB-MP323339HII1
CSB-EP323339HII1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Calcium-dependent serine proteinase, partial CSB-YP323340MRG
CSB-EP323340MRG
CSB-BP323340MRG
CSB-MP323340MRG
CSB-EP323340MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Apus apus Hemoglobin subunit alpha-A (HBAA) CSB-YP323341AMY
CSB-EP323341AMY
CSB-BP323341AMY
CSB-MP323341AMY
CSB-EP323341AMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Transcriptional regulator Myc-B (myc-b) CSB-YP323342XBE
CSB-EP323342XBE
CSB-BP323342XBE
CSB-MP323342XBE
CSB-EP323342XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Lysine--tRNA ligase, cytoplasmic (KRS1), partial CSB-YP323343SVG
CSB-EP323343SVG
CSB-BP323343SVG
CSB-MP323343SVG
CSB-EP323343SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem Q (B) protein, partial CSB-YP323344PSD1
CSB-EP323344PSD1
CSB-BP323344PSD1
CSB-MP323344PSD1
CSB-EP323344PSD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabies virus Putative uncharacterized protein C (C) CSB-YP323345RAI
CSB-EP323345RAI
CSB-BP323345RAI
CSB-MP323345RAI
CSB-EP323345RAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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