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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptomycin 3'-kinase (aphE) CSB-YP323570SUW
CSB-EP323570SUW
CSB-BP323570SUW
CSB-MP323570SUW
CSB-EP323570SUW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Dual specificity protein kinase splA (splA), partial CSB-YP323571DKK
CSB-EP323571DKK
CSB-BP323571DKK
CSB-MP323571DKK
CSB-EP323571DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein serrate (Ser), partial CSB-YP323572DLU
CSB-EP323572DLU
CSB-BP323572DLU
CSB-MP323572DLU
CSB-EP323572DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Periplasmic [NiFe] hydrogenase large subunit (hydB), partial CSB-YP323573DDD
CSB-EP323573DDD
CSB-BP323573DDD
CSB-MP323573DDD
CSB-EP323573DDD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae ADP,ATP carrier protein 2 (PET9), partial CSB-YP323574SVG1
CSB-EP323574SVG1
CSB-BP323574SVG1
CSB-MP323574SVG1
CSB-EP323574SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Threonine--tRNA ligase 2 (thrZ), partial CSB-YP323575BRJ
CSB-EP323575BRJ
CSB-BP323575BRJ
CSB-MP323575BRJ
CSB-EP323575BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloferax volcanii Tryptophan synthase beta chain (trpB) CSB-YP323577HTU
CSB-EP323577HTU
CSB-BP323577HTU
CSB-MP323577HTU
CSB-EP323577HTU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkanal monooxygenase alpha chain (luxA) CSB-YP323578KAR
CSB-EP323578KAR
CSB-BP323578KAR
CSB-MP323578KAR
CSB-EP323578KAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Plasmid-derived single-stranded DNA-binding protein (ssbF) CSB-YP323579ENV
CSB-BP323579ENV
CSB-MP323579ENV
CSB-EP323579ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter aerogenes Urease accessory protein UreF (ureF) CSB-YP323580EKN
CSB-EP323580EKN
CSB-BP323580EKN
CSB-MP323580EKN
CSB-EP323580EKN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ferredoxin-1 (suaB) CSB-YP323581SOJ
CSB-EP323581SOJ
CSB-BP323581SOJ
CSB-MP323581SOJ
CSB-EP323581SOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Isoleucine--tRNA ligase 1 (ileS1), partial CSB-YP323582FFT
CSB-EP323582FFT
CSB-BP323582FFT
CSB-MP323582FFT
CSB-EP323582FFT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tobacco mild green mosaic virus Replicase large subunit, partial CSB-YP323583TEY
CSB-EP323583TEY
CSB-BP323583TEY
CSB-MP323583TEY
CSB-EP323583TEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized 6.6 kDa protein CSB-YP323584ENL
CSB-EP323584ENL
CSB-BP323584ENL
CSB-MP323584ENL
CSB-EP323584ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sheeppox virus Protein T3A (T3A) CSB-YP323586SFV
CSB-EP323586SFV
CSB-BP323586SFV
CSB-MP323586SFV
CSB-EP323586SFV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable A-factor biosynthesis enzyme (afsA) CSB-YP323587SUW
CSB-EP323587SUW
CSB-BP323587SUW
CSB-MP323587SUW
CSB-EP323587SUW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Transposase for transposon Tn552 CSB-YP323588FKZ
CSB-BP323588FKZ
CSB-MP323588FKZ
CSB-EP323588FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse H-2 class II histocompatibility antigen, I-E beta chain (H2-Eb1), partial CSB-YP323589MO
CSB-EP323589MO
CSB-BP323589MO
CSB-MP323589MO
CSB-EP323589MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Tyrosine-protein kinase receptor torso (tor), partial CSB-YP323590DLU
CSB-EP323590DLU
CSB-BP323590DLU
CSB-MP323590DLU
CSB-EP323590DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Mitochondrial ATPase complex subunit ATP10 (ATP10) CSB-YP323591SVG
CSB-EP323591SVG
CSB-BP323591SVG
CSB-MP323591SVG
CSB-EP323591SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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