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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Methanococcus vannielii 50S ribosomal protein L1P (rpl1p) CSB-YP323653MNS
CSB-EP323653MNS
CSB-BP323653MNS
CSB-MP323653MNS
CSB-EP323653MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype B Gag polyprotein (gag), partial CSB-YP323654HMD
CSB-EP323654HMD
CSB-BP323654HMD
CSB-MP323654HMD
CSB-EP323654HMD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schistosoma mansoni 20 kDa calcium-binding protein (SM20) CSB-YP323655SXQ
CSB-EP323655SXQ
CSB-BP323655SXQ
CSB-MP323655SXQ
CSB-EP323655SXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Apis mellifera Homeobox protein H40 CSB-YP323656DNK
CSB-EP323656DNK
CSB-BP323656DNK
CSB-MP323656DNK
CSB-EP323656DNK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Histone H1C CSB-YP323657XBE
CSB-EP323657XBE
CSB-BP323657XBE
CSB-MP323657XBE
CSB-EP323657XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium 2-isopropylmalate synthase (leuA), partial CSB-YP323658SXB
CSB-EP323658SXB
CSB-BP323658SXB
CSB-MP323658SXB
CSB-EP323658SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spiroplasma virus SpV1-R8A2 B Putative integrase ORF3 (ORF3) CSB-YP323659SJZ
CSB-EP323659SJZ
CSB-BP323659SJZ
CSB-MP323659SJZ
CSB-EP323659SJZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spiroplasma virus SpV1-R8A2 B Uncharacterized protein ORF12 (ORF12), partial CSB-YP323660SJZ
CSB-EP323660SJZ
CSB-BP323660SJZ
CSB-MP323660SJZ
CSB-EP323660SJZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fowlpox virus Core protein VP8 (FPV131) CSB-YP323661FEY
CSB-EP323661FEY
CSB-BP323661FEY
CSB-MP323661FEY
CSB-EP323661FEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia rickettsii Outer membrane protein A (ompA), partial CSB-YP323662RNA
CSB-EP323662RNA
CSB-BP323662RNA
CSB-MP323662RNA
CSB-EP323662RNA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Flagellar assembly protein fliH (fliH) CSB-YP323663SXB
CSB-EP323663SXB
CSB-BP323663SXB
CSB-MP323663SXB
CSB-EP323663SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage fr Assembly protein (A) CSB-YP323664ECK
CSB-EP323664ECK
CSB-BP323664ECK
CSB-MP323664ECK
CSB-EP323664ECK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Putative per-hexamer repeat protein 4 (Phxr4) CSB-YP323665MO
CSB-EP323665MO
CSB-BP323665MO
CSB-MP323665MO
CSB-EP323665MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig SLA class II histocompatibility antigen, DQ haplotype D alpha chain, partial CSB-YP323666PI
CSB-EP323666PI
CSB-BP323666PI
CSB-MP323666PI
CSB-EP323666PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Plastocyanin, chloroplastic (PETE) CSB-YP323667EWE
CSB-EP323667EWE
CSB-BP323667EWE
CSB-MP323667EWE
CSB-EP323667EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Baseplate structural protein Gp53 (53) CSB-YP323668EDZ
CSB-EP323668EDZ
CSB-BP323668EDZ
CSB-MP323668EDZ
CSB-EP323668EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP323669ZAX
CSB-EP323669ZAX
CSB-BP323669ZAX
CSB-MP323669ZAX
CSB-EP323669ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Photosystem Q (B) protein 2, partial CSB-YP323670SSQ1
CSB-EP323670SSQ1
CSB-BP323670SSQ1
CSB-MP323670SSQ1
CSB-EP323670SSQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Guanine nucleotide-binding protein alpha-2 subunit (gpaB) CSB-YP323671DKK
CSB-EP323671DKK
CSB-BP323671DKK
CSB-MP323671DKK
CSB-EP323671DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Subtilisin-chymotrypsin inhibitor CI-1A CSB-YP323672HWQ
CSB-EP323672HWQ
CSB-BP323672HWQ
CSB-MP323672HWQ
CSB-EP323672HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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