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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Enterobacter agglomerans Pyruvate-flavodoxin oxidoreductase (nifJ), partial CSB-YP323943EKP
CSB-EP323943EKP
CSB-BP323943EKP
CSB-MP323943EKP
CSB-EP323943EKP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Envelope glycoprotein gp160 (env), partial CSB-YP323944HLE
CSB-EP323944HLE
CSB-BP323944HLE
CSB-MP323944HLE
CSB-EP323944HLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine immunodeficiency virus Virion infectivity factor (vif) CSB-YP323945BKB
CSB-EP323945BKB
CSB-BP323945BKB
CSB-MP323945BKB
CSB-EP323945BKB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus sp. Glucan 1,4-alpha-maltohexaosidase CSB-YP323946BBU
CSB-EP323946BBU
CSB-BP323946BBU
CSB-MP323946BBU
CSB-EP323946BBU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lantibiotic Pep5 (pepA) CSB-YP323947FLL
CSB-EP323947FLL
CSB-BP323947FLL
CSB-MP323947FLL
CSB-EP323947FLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoanaerobacter thermosulfurogenes Thermophilic beta-amylase, partial CSB-YP323948TCZ
CSB-EP323948TCZ
CSB-BP323948TCZ
CSB-MP323948TCZ
CSB-EP323948TCZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein iss (iss) CSB-YP323949ENL
CSB-EP323949ENL
CSB-BP323949ENL
CSB-MP323949ENL
CSB-EP323949ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium falciparum Merozoite surface antigen 2 (MSA2) CSB-YP323950PLT
CSB-EP323950PLT
CSB-BP323950PLT
CSB-MP323950PLT
CSB-EP323950PLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macrozoarces americanus Ice-structuring protein SP2 (HPLC 11) CSB-YP323951MAQ
CSB-EP323951MAQ
CSB-BP323951MAQ
CSB-MP323951MAQ
CSB-EP323951MAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Taenia ovis 45 kDa antigen CSB-YP323952TAK
CSB-EP323952TAK
CSB-BP323952TAK
CSB-MP323952TAK
CSB-EP323952TAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ethanolamine ammonia-lyase light chain (eutC) CSB-YP323953ENV
CSB-BP323953ENV
CSB-MP323953ENV
CSB-EP323953ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Donkey Beta-lactoglobulin-2 (LGB2) CSB-YP323954DK
CSB-EP323954DK
CSB-BP323954DK
CSB-MP323954DK
CSB-EP323954DK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage P1 Antirepressor protein 1 (ant) CSB-YP323955EDD
CSB-EP323955EDD
CSB-BP323955EDD
CSB-MP323955EDD
CSB-EP323955EDD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Cathelicidin-2 (CATHL2) CSB-YP323956BO
CSB-EP323956BO
CSB-BP323956BO
CSB-MP323956BO
CSB-EP323956BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beta-galactosidase bgaB (bgaB), partial CSB-YP323957GBI
CSB-EP323957GBI
CSB-BP323957GBI
CSB-MP323957GBI
CSB-EP323957GBI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyruvate kinase (pyk) CSB-YP323958FKO
CSB-EP323958FKO
CSB-BP323958FKO
CSB-MP323958FKO
CSB-EP323958FKO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Guanylate cyclase soluble subunit alpha-1 (GUCY1A1), partial CSB-YP323959BO
CSB-EP323959BO
CSB-BP323959BO
CSB-MP323959BO
CSB-EP323959BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T3 Major capsid protein 10A (10) CSB-YP323960EDY
CSB-EP323960EDY
CSB-BP323960EDY
CSB-MP323960EDY
CSB-EP323960EDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Hemagglutinin (HA), partial CSB-YP323961IDR
CSB-EP323961IDR
CSB-BP323961IDR
CSB-MP323961IDR
CSB-EP323961IDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Type-1 restriction enzyme EcoAI specificity protein (hsdS) CSB-YP323962ENL
CSB-EP323962ENL
CSB-BP323962ENL
CSB-MP323962ENL
CSB-EP323962ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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