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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae Nitrogen permease reactivator protein (NPR1), partial CSB-YP324355SVG
CSB-EP324355SVG
CSB-BP324355SVG
CSB-MP324355SVG
CSB-EP324355SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized transporter SLY41 (SLY41), partial CSB-YP324356SVG
CSB-EP324356SVG
CSB-BP324356SVG
CSB-MP324356SVG
CSB-EP324356SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase VPS15 (VPS15), partial CSB-YP324357SVG
CSB-EP324357SVG
CSB-BP324357SVG
CSB-MP324357SVG
CSB-EP324357SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellulosimicrobium cellulans Glucan endo-1,3-beta-glucosidase, partial CSB-YP324358CFT
CSB-EP324358CFT
CSB-BP324358CFT
CSB-MP324358CFT
CSB-EP324358CFT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 69 kDa paraflagellar rod protein (PFRA), partial CSB-YP324359TQV
CSB-EP324359TQV
CSB-BP324359TQV
CSB-MP324359TQV
CSB-EP324359TQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Canine adenovirus serotype 1 Early E3 22.2 kDa glycoprotein CSB-YP324360CCK
CSB-EP324360CCK
CSB-BP324360CCK
CSB-MP324360CCK
CSB-EP324360CCK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Capsule polysaccharide export inner-membrane protein BexB (bexB), partial CSB-YP324362HSZ1
CSB-EP324362HSZ1
CSB-BP324362HSZ1
CSB-MP324362HSZ1
CSB-EP324362HSZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desiccation-related protein clone PCC27-04 CSB-YP324363DXS
CSB-EP324363DXS
CSB-BP324363DXS
CSB-MP324363DXS
CSB-EP324363DXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Abscisic acid and environmental stress-inducible protein TAS14 (TAS14) CSB-YP324364SZY
CSB-EP324364SZY
CSB-BP324364SZY
CSB-MP324364SZY
CSB-EP324364SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carthamus tinctorius Acyl-[acyl-carrier-protein] desaturase, chloroplastic CSB-YP324365CEH
CSB-EP324365CEH
CSB-BP324365CEH
CSB-MP324365CEH
CSB-EP324365CEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:6 Flagellin A (flaA), partial CSB-YP324366CWG
CSB-EP324366CWG
CSB-BP324366CWG
CSB-MP324366CWG
CSB-EP324366CWG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetogenium kivui Cell surface protein, partial CSB-YP324367ABB
CSB-EP324367ABB
CSB-BP324367ABB
CSB-MP324367ABB
CSB-EP324367ABB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris CRP-like protein Clp (clp) CSB-YP324368XAY
CSB-EP324368XAY
CSB-BP324368XAY
CSB-MP324368XAY
CSB-EP324368XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. syringae Outer membrane porin F (oprF) CSB-YP324369PWD
CSB-EP324369PWD
CSB-BP324369PWD
CSB-MP324369PWD
CSB-EP324369PWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Calliphora vicina Opsin Rh1 (NINAE), partial CSB-YP324370CAX1
CSB-EP324370CAX1
CSB-BP324370CAX1
CSB-MP324370CAX1
CSB-EP324370CAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Tubulin alpha-3 chain (TUBA3) CSB-YP324371ZAX
CSB-EP324371ZAX
CSB-BP324371ZAX
CSB-MP324371ZAX
CSB-EP324371ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mucor circinelloides f. lusitanicus Ras-like protein 1 (RAS1) CSB-YP324372MIW
CSB-EP324372MIW
CSB-BP324372MIW
CSB-MP324372MIW
CSB-EP324372MIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Cystatin-related protein 2 (Crp2) CSB-YP324373RA
CSB-EP324373RA
CSB-BP324373RA
CSB-MP324373RA
CSB-EP324373RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Poa pratensis Pollen allergen KBG 60 CSB-YP324374PNP
CSB-EP324374PNP
CSB-BP324374PNP
CSB-MP324374PNP
CSB-EP324374PNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Bacteriocin helveticin-J (hlv) CSB-YP324375LMP
CSB-EP324375LMP
CSB-BP324375LMP
CSB-MP324375LMP
CSB-EP324375LMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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