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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Feline immunodeficiency virus Pol polyprotein (pol) CSB-YP323900FAM
CSB-EP323900FAM
CSB-BP323900FAM
CSB-MP323900FAM
CSB-EP323900FAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Apis mellifera Elongation factor 1-alpha CSB-YP323901DNK
CSB-EP323901DNK
CSB-BP323901DNK
CSB-MP323901DNK
CSB-EP323901DNK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus Reaction center protein H chain (puhA), partial CSB-YP323902RLC1
CSB-EP323902RLC1
CSB-BP323902RLC1
CSB-MP323902RLC1
CSB-EP323902RLC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Chemokine-binding protein (C23L) CSB-YP323903VAG
CSB-EP323903VAG
CSB-BP323903VAG
CSB-MP323903VAG
CSB-EP323903VAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus Nitrogenase iron-molybdenum cofactor biosynthesis protein NifN (nifN) CSB-YP323904RLC
CSB-EP323904RLC
CSB-BP323904RLC
CSB-MP323904RLC
CSB-EP323904RLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helianthus annuus 11S globulin seed storage protein G3 (HAG3) CSB-YP323905HUO
CSB-EP323905HUO
CSB-BP323905HUO
CSB-MP323905HUO
CSB-EP323905HUO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Japanese encephalitis virus Genome polyprotein, partial CSB-YP323906JAE
CSB-EP323906JAE
CSB-BP323906JAE
CSB-MP323906JAE
CSB-EP323906JAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Atriplex patula Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP323907AQV
CSB-EP323907AQV
CSB-BP323907AQV
CSB-MP323907AQV
CSB-EP323907AQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S3, chloroplastic (rps3) CSB-YP323908EHU
CSB-EP323908EHU
CSB-BP323908EHU
CSB-MP323908EHU
CSB-EP323908EHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carassius auratus Ig heavy chain V region 3 CSB-YP323910DQG
CSB-EP323910DQG
CSB-BP323910DQG
CSB-MP323910DQG
CSB-EP323910DQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia phage 1 Internal scaffolding protein VP3 (ORF3) CSB-YP323911CIV
CSB-EP323911CIV
CSB-BP323911CIV
CSB-MP323911CIV
CSB-EP323911CIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Commelina yellow mottle virus Polyprotein P3, partial CSB-YP323912CNO
CSB-EP323912CNO
CSB-BP323912CNO
CSB-MP323912CNO
CSB-EP323912CNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum 17.1 kDa class II heat shock protein (HSP17.7) CSB-YP323913EWE
CSB-EP323913EWE
CSB-BP323913EWE
CSB-MP323913EWE
CSB-EP323913EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytolysin secretion protein (vvhB) CSB-YP323914VFI
CSB-EP323914VFI
CSB-BP323914VFI
CSB-MP323914VFI
CSB-EP323914VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pilin gene-inverting protein (piv) CSB-YP323915MIB
CSB-EP323915MIB
CSB-BP323915MIB
CSB-MP323915MIB
CSB-EP323915MIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium falciparum Merozoite surface antigen 2, allelic form 2 (MSA2) CSB-YP323916PLW
CSB-EP323916PLW
CSB-BP323916PLW
CSB-MP323916PLW
CSB-EP323916PLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus tenax virus 1 Uncharacterized 14.8 kDa protein CSB-YP323918TDO
CSB-EP323918TDO
CSB-BP323918TDO
CSB-MP323918TDO
CSB-EP323918TDO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus tenax virus 1 Uncharacterized 8.9 kDa protein CSB-YP323919TDO
CSB-EP323919TDO
CSB-BP323919TDO
CSB-MP323919TDO
CSB-EP323919TDO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus tenax virus 1 Uncharacterized 11.5 kDa protein CSB-YP323920TDO
CSB-EP323920TDO
CSB-BP323920TDO
CSB-MP323920TDO
CSB-EP323920TDO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus tenax virus 1 Uncharacterized 6.1 kDa protein CSB-YP323921TDO
CSB-EP323921TDO
CSB-BP323921TDO
CSB-MP323921TDO
CSB-EP323921TDO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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