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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana 17.4 kDa class I heat shock protein (HSP17.4A) CSB-YP324766DOA
CSB-EP324766DOA
CSB-BP324766DOA
CSB-MP324766DOA
CSB-EP324766DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Secale cereale Photosystem II reaction center protein J (psbJ), partial CSB-YP324767STH
CSB-EP324767STH
CSB-BP324767STH
CSB-MP324767STH
CSB-EP324767STH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Nitrogenase iron protein (nifH1) CSB-YP324768RKX
CSB-EP324768RKX
CSB-BP324768RKX
CSB-MP324768RKX
CSB-EP324768RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T6 DNA ligase (30) CSB-YP324769EEA
CSB-EP324769EEA
CSB-BP324769EEA
CSB-MP324769EEA
CSB-EP324769EEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila miranda Phosrestin-1 (Arr2) CSB-YP324770DLX
CSB-EP324770DLX
CSB-BP324770DLX
CSB-MP324770DLX
CSB-EP324770DLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine adenovirus B serotype 3 Adenain CSB-YP324771BVG
CSB-EP324771BVG
CSB-BP324771BVG
CSB-MP324771BVG
CSB-EP324771BVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaseolus vulgaris Phenylalanine ammonia-lyase class 2, partial CSB-YP324772EUN
CSB-EP324772EUN
CSB-BP324772EUN
CSB-MP324772EUN
CSB-EP324772EUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae ADP-ribosylation factor 2 (ARF2) CSB-YP324773SVG
CSB-EP324773SVG
CSB-BP324773SVG
CSB-MP324773SVG
CSB-EP324773SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem II reaction center protein L (psbL), partial CSB-YP324774DZX
CSB-EP324774DZX
CSB-BP324774DZX
CSB-MP324774DZX
CSB-EP324774DZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L2, chloroplastic (rpl2) CSB-YP324775EHU
CSB-EP324775EHU
CSB-BP324775EHU
CSB-MP324775EHU
CSB-EP324775EHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Basic endochitinase B (CHI-B) CSB-YP324776DOA
CSB-EP324776DOA
CSB-BP324776DOA
CSB-MP324776DOA
CSB-EP324776DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Platynereis dumerilii Histone H2A CSB-YP324777PMX
CSB-EP324777PMX
CSB-BP324777PMX
CSB-MP324777PMX
CSB-EP324777PMX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia phage 1 Replication-associated protein ORF4 (ORF4) CSB-YP324778CIV
CSB-EP324778CIV
CSB-BP324778CIV
CSB-MP324778CIV
CSB-EP324778CIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Commelina yellow mottle virus Uncharacterized 23 kDa protein CSB-YP324779CNO
CSB-EP324779CNO
CSB-BP324779CNO
CSB-MP324779CNO
CSB-EP324779CNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysinibacillus sphaericus Biotin synthase (bioB) CSB-YP324780LRU
CSB-EP324780LRU
CSB-BP324780LRU
CSB-MP324780LRU
CSB-EP324780LRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium sp. Creatinase CSB-YP324781FBY
CSB-EP324781FBY
CSB-BP324781FBY
CSB-MP324781FBY
CSB-EP324781FBY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Bifunctional transcriptional activator/DNA repair enzyme AdaA (adaA) CSB-YP324782BRJ
CSB-EP324782BRJ
CSB-BP324782BRJ
CSB-MP324782BRJ
CSB-EP324782BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mopeia virus Nucleoprotein (N), partial CSB-YP324783MHY
CSB-EP324783MHY
CSB-BP324783MHY
CSB-MP324783MHY
CSB-EP324783MHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum 22.7 kDa class IV heat shock protein (HSP22.7) CSB-YP324784EWE
CSB-EP324784EWE
CSB-BP324784EWE
CSB-MP324784EWE
CSB-EP324784EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio parahaemolyticus serotype O3:K6 Thermostable direct hemolysin 1 (tdh1) CSB-YP324785VFE
CSB-BP324785VFE
CSB-MP324785VFE
CSB-EP324785VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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