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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Carbon monoxide dehydrogenase small chain (cutS) CSB-YP323983PUE
CSB-EP323983PUE
CSB-BP323983PUE
CSB-MP323983PUE
CSB-EP323983PUE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Carbon monoxide dehydrogenase small chain (coxS) CSB-YP323984OED
CSB-EP323984OED
CSB-BP323984OED
CSB-MP323984OED
CSB-EP323984OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Hydrogenase-1 operon protein hyaE (hyaE) CSB-YP323985ENV
CSB-EP323985ENV
CSB-BP323985ENV
CSB-MP323985ENV
CSB-EP323985ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui 30S ribosomal protein S19e (rps19e) CSB-YP323986HTJ
CSB-EP323986HTJ
CSB-BP323986HTJ
CSB-MP323986HTJ
CSB-EP323986HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aipysurus laevis Short neurotoxin B CSB-YP323987AFL
CSB-EP323987AFL
CSB-BP323987AFL
CSB-MP323987AFL
CSB-EP323987AFL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clypeaster japonicus [Gln6]-mosact CSB-YP323988CMU
CSB-EP323988CMU
CSB-BP323988CMU
CSB-MP323988CMU
CSB-EP323988CMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Millerozyma farinosa Salt-mediated killer protoxin 1 (SMK1) CSB-YP323989MGS
CSB-EP323989MGS
CSB-BP323989MGS
CSB-MP323989MGS
CSB-EP323989MGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhyparobia maderae Leucokinin-6 CSB-YP323990REJ
CSB-EP323990REJ
CSB-BP323990REJ
CSB-MP323990REJ
CSB-EP323990REJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces exfoliatus 3-alpha- (or 20-beta)-hydroxysteroid dehydrogenase CSB-YP323991SOC
CSB-EP323991SOC
CSB-BP323991SOC
CSB-MP323991SOC
CSB-EP323991SOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tritirachium album Proteinase T (PROT) CSB-YP323992TIQ
CSB-EP323992TIQ
CSB-BP323992TIQ
CSB-MP323992TIQ
CSB-EP323992TIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant V-type ATP synthase beta chain (atpB) CSB-YP323993MSC
CSB-EP323993MSC
CSB-BP323993MSC
CSB-MP323993MSC
CSB-EP323993MSC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Myb-related protein Hv33 (MYB2) CSB-YP323994HWQ
CSB-EP323994HWQ
CSB-BP323994HWQ
CSB-MP323994HWQ
CSB-EP323994HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heat shock cognate HSP70 protein, partial CSB-YP323995TQV
CSB-EP323995TQV
CSB-BP323995TQV
CSB-MP323995TQV
CSB-EP323995TQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Rano class II histocompatibility antigen, B alpha chain (RT1-Ba), partial CSB-YP323996RA
CSB-EP323996RA
CSB-BP323996RA
CSB-MP323996RA
CSB-EP323996RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Ethylene-responsive proteinase inhibitor 1 CSB-YP323997SZY
CSB-EP323997SZY
CSB-BP323997SZY
CSB-MP323997SZY
CSB-EP323997SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alpha-amylase inhibitor Haim-2 CSB-YP323998SOL
CSB-EP323998SOL
CSB-BP323998SOL
CSB-MP323998SOL
CSB-EP323998SOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thiobacillus ferrooxidans Mobilization protein mobS (mobS) CSB-YP323999TOA
CSB-EP323999TOA
CSB-BP323999TOA
CSB-MP323999TOA
CSB-EP323999TOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Interleukin-4 (Il4) CSB-YP324000RA
CSB-EP324000RA
CSB-BP324000RA
CSB-MP324000RA
CSB-EP324000RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. lactis Nisin biosynthesis protein nisB (nisB), partial CSB-YP324001LNI
CSB-EP324001LNI
CSB-BP324001LNI
CSB-MP324001LNI
CSB-EP324001LNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Photosystem I reaction center subunit III (PSAF) CSB-YP324002EWE
CSB-EP324002EWE
CSB-BP324002EWE
CSB-MP324002EWE
CSB-EP324002EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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