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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli Sulfite reductase [NADPH] hemoprotein beta-component (cysI) CSB-YP324663ENV
CSB-EP324663ENV
CSB-BP324663ENV
CSB-MP324663ENV
CSB-EP324663ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate 5-kinase (proB) CSB-YP324664SYN
CSB-EP324664SYN
CSB-BP324664SYN
CSB-MP324664SYN
CSB-EP324664SYN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium loti N-acetylglucosaminyltransferase (nodC) CSB-YP324665RCU
CSB-EP324665RCU
CSB-BP324665RCU
CSB-MP324665RCU
CSB-EP324665RCU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Mannitol-specific phosphotransferase enzyme IIA component (mtlF) CSB-YP324666FLK
CSB-EP324666FLK
CSB-BP324666FLK
CSB-MP324666FLK
CSB-EP324666FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Seoul virus Envelope glycoprotein (GP), partial CSB-YP324667SFG1
CSB-EP324667SFG1
CSB-BP324667SFG1
CSB-MP324667SFG1
CSB-EP324667SFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Lysozyme C-1 (Lyz1) CSB-YP324669MO
CSB-EP324669MO
CSB-BP324669MO
CSB-MP324669MO
CSB-EP324669MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein traJ (traJ) CSB-YP324670ENL
CSB-EP324670ENL
CSB-BP324670ENL
CSB-MP324670ENL
CSB-EP324670ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP324671BRJ
CSB-EP324671BRJ
CSB-BP324671BRJ
CSB-MP324671BRJ
CSB-EP324671BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized 21 kDa protein in aldolase locus CSB-YP324672TQV
CSB-EP324672TQV
CSB-BP324672TQV
CSB-MP324672TQV
CSB-EP324672TQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Potassium voltage-gated channel protein Shab (Shab), partial CSB-YP324673DLU
CSB-EP324673DLU
CSB-BP324673DLU
CSB-MP324673DLU
CSB-EP324673DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Virginiamycin B lyase (vgb) CSB-YP324674FKZ
CSB-EP324674FKZ
CSB-BP324674FKZ
CSB-MP324674FKZ
CSB-EP324674FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Insertion element ISR1 uncharacterized 10 kDa protein A3 CSB-YP324675RCW
CSB-EP324675RCW
CSB-BP324675RCW
CSB-MP324675RCW
CSB-EP324675RCW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare C-hordein CSB-YP324676HWQ
CSB-EP324676HWQ
CSB-BP324676HWQ
CSB-MP324676HWQ
CSB-EP324676HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0380 protein yubP (yubP) CSB-YP324677ENV
CSB-EP324677ENV
CSB-BP324677ENV
CSB-MP324677ENV
CSB-EP324677ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri Invasin ipaC (ipaC) CSB-YP324678SZB
CSB-BP324678SZB
CSB-MP324678SZB
CSB-EP324678SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative uncharacterized protein ORF9 CSB-YP324679CMB
CSB-EP324679CMB
CSB-BP324679CMB
CSB-MP324679CMB
CSB-EP324679CMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cucumber mosaic virus Capsid protein (ORF3b) CSB-YP324680CUE
CSB-EP324680CUE
CSB-BP324680CUE
CSB-MP324680CUE
CSB-EP324680CUE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype A Envelope glycoprotein gp160 (env), partial CSB-YP324681HLX
CSB-EP324681HLX
CSB-BP324681HLX
CSB-MP324681HLX
CSB-EP324681HLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equine herpesvirus 1 Envelope glycoprotein B (gB), partial CSB-YP324682EFL
CSB-EP324682EFL
CSB-BP324682EFL
CSB-MP324682EFL
CSB-EP324682EFL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Putative endonuclease segE (segE) CSB-YP324683EDZ
CSB-EP324683EDZ
CSB-BP324683EDZ
CSB-MP324683EDZ
CSB-EP324683EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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