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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human parainfluenza 4b virus Non-structural protein V (P/V) CSB-YP324251HPL
CSB-EP324251HPL
CSB-BP324251HPL
CSB-MP324251HPL
CSB-EP324251HPL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans Protein spalt-accessory (sala) CSB-YP324252DMJ
CSB-EP324252DMJ
CSB-BP324252DMJ
CSB-MP324252DMJ
CSB-EP324252DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein spalt-accessory (sala) CSB-YP324253DLU
CSB-EP324253DLU
CSB-BP324253DLU
CSB-MP324253DLU
CSB-EP324253DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protobothrops flavoviridis Phospholipase A2 inhibitor subunit B CSB-YP324254EZB
CSB-EP324254EZB
CSB-BP324254EZB
CSB-MP324254EZB
CSB-EP324254EZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Probable peroxiredoxin (tsaA) CSB-YP324255HUV
CSB-EP324255HUV
CSB-BP324255HUV
CSB-MP324255HUV
CSB-EP324255HUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca sinica Hemoglobin subunit alpha-1/2 CSB-YP324256MND
CSB-EP324256MND
CSB-BP324256MND
CSB-MP324256MND
CSB-EP324256MND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 6-phosphofructokinase isozyme 2 CSB-YP324257TNT
CSB-EP324257TNT
CSB-BP324257TNT
CSB-MP324257TNT
CSB-EP324257TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Platichthys flesus Somatostatin-2 (sst2) CSB-YP324258EWX
CSB-EP324258EWX
CSB-BP324258EWX
CSB-MP324258EWX
CSB-EP324258EWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse V (D)J recombination-activating protein 2 CSB-YP324259MO
CSB-EP324259MO
CSB-BP324259MO
CSB-MP324259MO
CSB-EP324259MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Micrurus nigrocinctus Phospholipase A2 isozyme 3 CSB-YP324260MGO
CSB-EP324260MGO
CSB-BP324260MGO
CSB-MP324260MGO
CSB-EP324260MGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycolicibacterium smegmatis Lactate 2-monooxygenase CSB-YP324261MVW
CSB-EP324261MVW
CSB-BP324261MVW
CSB-MP324261MVW
CSB-EP324261MVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Balanus nubilis Troponin C, isoform 2 CSB-YP324262BEE
CSB-EP324262BEE
CSB-BP324262BEE
CSB-MP324262BEE
CSB-EP324262BEE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pleurotus ostreatus Veratryl alcohol oxidase CSB-YP324263EXB
CSB-EP324263EXB
CSB-BP324263EXB
CSB-MP324263EXB
CSB-EP324263EXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sheep Uterine milk protein CSB-YP324264SH
CSB-EP324264SH
CSB-BP324264SH
CSB-MP324264SH
CSB-EP324264SH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Manduca sexta Diuretic hormone 1 CSB-YP324265MPV
CSB-EP324265MPV
CSB-BP324265MPV
CSB-MP324265MPV
CSB-EP324265MPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter aerogenes Chemotaxis protein methyltransferase (cheR) CSB-YP324266EKO
CSB-EP324266EKO
CSB-BP324266EKO
CSB-MP324266EKO
CSB-EP324266EKO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Guanine nucleotide exchange factor SRM1 (SRM1) CSB-YP324267SVG
CSB-EP324267SVG
CSB-BP324267SVG
CSB-MP324267SVG
CSB-EP324267SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sclerotinia sclerotiorum Endoglucanase 1 CSB-YP324268SDU
CSB-EP324268SDU
CSB-BP324268SDU
CSB-MP324268SDU
CSB-EP324268SDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Crystal protein (cryS), partial CSB-YP324269DKK
CSB-EP324269DKK
CSB-BP324269DKK
CSB-MP324269DKK
CSB-EP324269DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Variant surface glycoprotein MVAT5 CSB-YP324270TQX
CSB-EP324270TQX
CSB-BP324270TQX
CSB-MP324270TQX
CSB-EP324270TQX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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