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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Fe (3+)-binding periplasmic protein CSB-YP324186SYN
CSB-EP324186SYN
CSB-BP324186SYN
CSB-MP324186SYN
CSB-EP324186SYN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fe (3+) ions import ATP-binding protein FbpC CSB-YP324187SYN
CSB-EP324187SYN
CSB-BP324187SYN
CSB-MP324187SYN
CSB-EP324187SYN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Squirrel monkey retrovirus Envelope glycoprotein (env), partial CSB-YP324188SKS
CSB-EP324188SKS
CSB-BP324188SKS
CSB-MP324188SKS
CSB-EP324188SKS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage P22 Uncharacterized 22.8 kDa protein in gp15-gp3 intergenic region CSB-YP324189EDG
CSB-EP324189EDG
CSB-BP324189EDG
CSB-MP324189EDG
CSB-EP324189EDG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transposon Tn1331 resolvase (tnpR) CSB-YP324191KBG
CSB-EP324191KBG
CSB-BP324191KBG
CSB-MP324191KBG
CSB-EP324191KBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Polymerase basic protein 2 (PB2), partial CSB-YP324192IAZ
CSB-EP324192IAZ
CSB-BP324192IAZ
CSB-MP324192IAZ
CSB-EP324192IAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Nucleoprotein (NP) CSB-YP324193IAZ
CSB-EP324193IAZ
CSB-BP324193IAZ
CSB-MP324193IAZ
CSB-EP324193IAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hortulanus murine leukemia virus Gag polyprotein (gag), partial CSB-YP324194HHZ
CSB-EP324194HHZ
CSB-BP324194HHZ
CSB-MP324194HHZ
CSB-EP324194HHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus phage HP1 Integrase (int) CSB-YP324195HAN
CSB-EP324195HAN
CSB-BP324195HAN
CSB-MP324195HAN
CSB-EP324195HAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mono (ADP-ribosyl)transferase SpvB, partial CSB-YP324196SWX
CSB-EP324196SWX
CSB-BP324196SWX
CSB-MP324196SWX
CSB-EP324196SWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis DNA translocase SpoIIIE (spoIIIE), partial CSB-YP324197BRJ1
CSB-EP324197BRJ1
CSB-BP324197BRJ1
CSB-MP324197BRJ1
CSB-EP324197BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 30S ribosomal protein S3 (rpsC) CSB-YP324198BRJ
CSB-EP324198BRJ
CSB-BP324198BRJ
CSB-MP324198BRJ
CSB-EP324198BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 30S ribosomal protein S6 (rpsF) CSB-YP324199BRJ
CSB-EP324199BRJ
CSB-BP324199BRJ
CSB-MP324199BRJ
CSB-EP324199BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 30S ribosomal protein S12 (rpsL) CSB-YP324200BRJ
CSB-EP324200BRJ
CSB-BP324200BRJ
CSB-MP324200BRJ
CSB-EP324200BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 30S ribosomal protein S18 (rpsR) CSB-YP324201BRJ
CSB-EP324201BRJ
CSB-BP324201BRJ
CSB-MP324201BRJ
CSB-EP324201BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 30S ribosomal protein S20 (rpsT) CSB-YP324202BRJ
CSB-EP324202BRJ
CSB-BP324202BRJ
CSB-MP324202BRJ
CSB-EP324202BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Disulfide bond formation protein B 1 (dsbB1), partial CSB-YP324203EZX1
CSB-EP324203EZX1
CSB-BP324203EZX1
CSB-MP324203EZX1
CSB-EP324203EZX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Homing endonuclease I-DmoI CSB-YP324204DDJ
CSB-EP324204DDJ
CSB-BP324204DDJ
CSB-MP324204DDJ
CSB-EP324204DDJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli ATP-dependent RNA helicase SrmB (srmB) CSB-YP324205ENV
CSB-EP324205ENV
CSB-BP324205ENV
CSB-MP324205ENV
CSB-EP324205ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L14, chloroplastic (rpl14) CSB-YP324206EHU
CSB-EP324206EHU
CSB-BP324206EHU
CSB-MP324206EHU
CSB-EP324206EHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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