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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Escherichia coli Ribonuclease E (rne), partial CSB-BP324207ENV
CSB-MP324207ENV
CSB-EP324207ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein scabrous (sca), partial CSB-YP324208DLU
CSB-EP324208DLU
CSB-BP324208DLU
CSB-MP324208DLU
CSB-EP324208DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Synaptotagmin 1 (Syt1), partial CSB-YP324209DLU
CSB-EP324209DLU
CSB-BP324209DLU
CSB-MP324209DLU
CSB-EP324209DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human parainfluenza 4a virus Hemagglutinin-neuraminidase (HN), partial CSB-YP324210HPK1
CSB-EP324210HPK1
CSB-BP324210HPK1
CSB-MP324210HPK1
CSB-EP324210HPK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Malate dehydrogenase [NADP], chloroplastic CSB-YP324211EWE
CSB-EP324211EWE
CSB-BP324211EWE
CSB-MP324211EWE
CSB-EP324211EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe ATP synthase subunit 9, mitochondrial (atp9), partial CSB-YP324212SXV1
CSB-EP324212SXV1
CSB-BP324212SXV1
CSB-MP324212SXV1
CSB-EP324212SXV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces thermotolerans 4'-mycarosyl isovaleryl-CoA transferase (carE) CSB-YP324213SQC
CSB-EP324213SQC
CSB-BP324213SQC
CSB-MP324213SQC
CSB-EP324213SQC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein CBP3, mitochondrial (CBP3), partial CSB-YP324214SVG1
CSB-EP324214SVG1
CSB-BP324214SVG1
CSB-MP324214SVG1
CSB-EP324214SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Peptidyl-prolyl cis-trans isomerase (CYP) CSB-YP324215SZY
CSB-EP324215SZY
CSB-BP324215SZY
CSB-MP324215SZY
CSB-EP324215SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coturnix coturnix japonica Myoblast determination protein 1 homolog (MYOD1) CSB-YP324216DXJ
CSB-EP324216DXJ
CSB-BP324216DXJ
CSB-MP324216DXJ
CSB-EP324216DXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio fischeri Yellow fluorescent protein (luxY) CSB-YP324217VDZ
CSB-EP324217VDZ
CSB-BP324217VDZ
CSB-MP324217VDZ
CSB-EP324217VDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T6 Major prohead-scaffolding core protein Gp22 (22) CSB-YP324219EEA
CSB-EP324219EEA
CSB-BP324219EEA
CSB-MP324219EEA
CSB-EP324219EEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Uncharacterized protein IRL14 CSB-YP324220HWV
CSB-EP324220HWV
CSB-BP324220HWV
CSB-MP324220HWV
CSB-EP324220HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein E2 (E2L), partial CSB-YP324221VAI
CSB-EP324221VAI
CSB-BP324221VAI
CSB-MP324221VAI
CSB-EP324221VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein E5 (VACWR061) CSB-YP324222VAI
CSB-EP324222VAI
CSB-BP324222VAI
CSB-MP324222VAI
CSB-EP324222VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A RNA-directed RNA polymerase, partial CSB-YP324223RGK
CSB-EP324223RGK
CSB-BP324223RGK
CSB-MP324223RGK
CSB-EP324223RGK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Nerve growth factor (ngf) CSB-YP324224XBE
CSB-EP324224XBE
CSB-BP324224XBE
CSB-MP324224XBE
CSB-EP324224XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Spore wall maturation protein DIT1 (DIT1), partial CSB-YP324225SVG
CSB-EP324225SVG
CSB-BP324225SVG
CSB-MP324225SVG
CSB-EP324225SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa High-affinity branched-chain amino acid transport system permease protein BraD (braD), partial CSB-YP324226EZX
CSB-EP324226EZX
CSB-BP324226EZX
CSB-MP324226EZX
CSB-EP324226EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cobyrinic acid A,C-diamide synthase (cobB) CSB-YP324227PUH
CSB-EP324227PUH
CSB-BP324227PUH
CSB-MP324227PUH
CSB-EP324227PUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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