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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Agrobacterium fabrum T-DNA border endonuclease virD2 (virD2) CSB-YP324724AYS
CSB-EP324724AYS
CSB-BP324724AYS
CSB-MP324724AYS
CSB-EP324724AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Artemia sp. Actin, clone 211 CSB-YP324725AOF
CSB-EP324725AOF
CSB-BP324725AOF
CSB-MP324725AOF
CSB-EP324725AOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bluetongue virus 17 Core protein VP7 (S7) CSB-YP324726BHU
CSB-EP324726BHU
CSB-BP324726BHU
CSB-MP324726BHU
CSB-EP324726BHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coturnix coturnix japonica Histone H5 CSB-YP324727DXJ
CSB-EP324727DXJ
CSB-BP324727DXJ
CSB-MP324727DXJ
CSB-EP324727DXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea Putative 50S ribosomal protein L23, chloroplastic (rpl23) CSB-YP324729FKI
CSB-EP324729FKI
CSB-BP324729FKI
CSB-MP324729FKI
CSB-EP324729FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Artocarpus integer Agglutinin alpha chain CSB-YP324730AOY
CSB-EP324730AOY
CSB-BP324730AOY
CSB-MP324730AOY
CSB-EP324730AOY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maclura pomifera Agglutinin beta-1 chain CSB-YP324731MAD
CSB-EP324731MAD
CSB-BP324731MAD
CSB-MP324731MAD
CSB-EP324731MAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage HK022 Regulatory protein CIII (CIII) CSB-YP324732ECN
CSB-EP324732ECN
CSB-BP324732ECN
CSB-MP324732ECN
CSB-EP324732ECN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cerastes vipera Thrombin-like enzyme cerastobin CSB-YP324733CGJ
CSB-EP324733CGJ
CSB-BP324733CGJ
CSB-MP324733CGJ
CSB-EP324733CGJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Gastrula zinc finger protein XlCGF16.1 CSB-YP324734XBE
CSB-EP324734XBE
CSB-BP324734XBE
CSB-MP324734XBE
CSB-EP324734XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Gastrula zinc finger protein xLCGF3.1 CSB-YP324735XBE
CSB-EP324735XBE
CSB-BP324735XBE
CSB-MP324735XBE
CSB-EP324735XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Gastrula zinc finger protein XlCGF48.2, partial CSB-YP324736XBE
CSB-EP324736XBE
CSB-BP324736XBE
CSB-MP324736XBE
CSB-EP324736XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Gastrula zinc finger protein XlCGF53.1, partial CSB-YP324737XBE
CSB-EP324737XBE
CSB-BP324737XBE
CSB-MP324737XBE
CSB-EP324737XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Gastrula zinc finger protein XlCGF67.1 CSB-YP324738XBE
CSB-EP324738XBE
CSB-BP324738XBE
CSB-MP324738XBE
CSB-EP324738XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Gastrula zinc finger protein XlCGF7.1 CSB-YP324739XBE
CSB-EP324739XBE
CSB-BP324739XBE
CSB-MP324739XBE
CSB-EP324739XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Oocyte zinc finger protein XlCOF19 CSB-YP324740XBE
CSB-EP324740XBE
CSB-BP324740XBE
CSB-MP324740XBE
CSB-EP324740XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Oocyte zinc finger protein XlCOF22 CSB-YP324741XBE
CSB-EP324741XBE
CSB-BP324741XBE
CSB-MP324741XBE
CSB-EP324741XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fimbrial protein (pilA) CSB-YP324742EZW
CSB-EP324742EZW
CSB-BP324742EZW
CSB-MP324742EZW
CSB-EP324742EZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tellurium resistance protein terA (terA) CSB-YP324743AGP
CSB-EP324743AGP
CSB-BP324743AGP
CSB-MP324743AGP
CSB-EP324743AGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP324744DPS
CSB-EP324744DPS
CSB-BP324744DPS
CSB-MP324744DPS
CSB-EP324744DPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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