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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Protein RecA (recA) CSB-YP324684MSW
CSB-EP324684MSW
CSB-BP324684MSW
CSB-MP324684MSW
CSB-EP324684MSW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype A Protein Rev (rev) CSB-YP324685HLU
CSB-EP324685HLU
CSB-BP324685HLU
CSB-MP324685HLU
CSB-EP324685HLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype A Virion infectivity factor (vif) CSB-YP324686HLU
CSB-EP324686HLU
CSB-BP324686HLU
CSB-MP324686HLU
CSB-EP324686HLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Kinesin-like protein Nod (nod), partial CSB-YP324687DLU
CSB-EP324687DLU
CSB-BP324687DLU
CSB-MP324687DLU
CSB-EP324687DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Thyroid hormone receptor beta-A (thrb-a) CSB-YP324688XBE
CSB-EP324688XBE
CSB-BP324688XBE
CSB-MP324688XBE
CSB-EP324688XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Autographa californica nuclear polyhedrosis virus DNA polymerase (POL), partial CSB-YP324689ARA
CSB-EP324689ARA
CSB-BP324689ARA
CSB-MP324689ARA
CSB-EP324689ARA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus 65 kDa phosphoprotein (UL83), partial CSB-YP324690HWX
CSB-EP324690HWX
CSB-BP324690HWX
CSB-MP324690HWX
CSB-EP324690HWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Glycerol uptake facilitator protein (glpF), partial CSB-YP324691BRJ1
CSB-EP324691BRJ1
CSB-BP324691BRJ1
CSB-MP324691BRJ1
CSB-EP324691BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 53 kDa membrane antigen A (tdpA) CSB-YP324692THQ
CSB-EP324692THQ
CSB-BP324692THQ
CSB-MP324692THQ
CSB-EP324692THQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila pseudoobscura pseudoobscura Glucose dehydrogenase [acceptor] (Gld), partial CSB-YP324693DME
CSB-EP324693DME
CSB-BP324693DME
CSB-MP324693DME
CSB-EP324693DME-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pectobacterium carotovorum subsp. carotovorum Endo-polygalacturonase (peh) CSB-YP324694ESP
CSB-EP324694ESP
CSB-BP324694ESP
CSB-MP324694ESP
CSB-EP324694ESP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Phosphatidylglycerophosphatase A (pgpA), partial CSB-YP324695ENV1
CSB-EP324695ENV1
CSB-BP324695ENV1
CSB-MP324695ENV1
CSB-EP324695ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Oxygen-evolving enhancer protein 2-2, chloroplastic (PSBP2) CSB-YP324696NHE
CSB-EP324696NHE
CSB-BP324696NHE
CSB-MP324696NHE
CSB-EP324696NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Potato virus Y Genome polyprotein, partial CSB-YP324697PRK
CSB-EP324697PRK
CSB-BP324697PRK
CSB-MP324697PRK
CSB-EP324697PRK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Artemia salina Ras-like protein CSB-YP324698AOE
CSB-EP324698AOE
CSB-BP324698AOE
CSB-MP324698AOE
CSB-EP324698AOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sialidase 85-1.2 (SA85-1.2) CSB-YP324699TQY
CSB-EP324699TQY
CSB-BP324699TQY
CSB-MP324699TQY
CSB-EP324699TQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brassica napus Photosystem Q (B) protein, partial CSB-YP324700BWD1
CSB-EP324700BWD1
CSB-BP324700BWD1
CSB-MP324700BWD1
CSB-EP324700BWD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter aerogenes Urease subunit beta (ureB) CSB-YP324701EKN
CSB-EP324701EKN
CSB-BP324701EKN
CSB-MP324701EKN
CSB-EP324701EKN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dendroaspis angusticeps Muscarinic toxin 2 CSB-YP324702DBG
CSB-EP324702DBG
CSB-BP324702DBG
CSB-MP324702DBG
CSB-EP324702DBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equine herpesvirus 4 Immediate-early protein IE68 CSB-YP324703EFR
CSB-EP324703EFR
CSB-BP324703EFR
CSB-MP324703EFR
CSB-EP324703EFR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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