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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Probable transport system permease protein nifC (nifC), partial CSB-YP324745CMA1
CSB-EP324745CMA1
CSB-BP324745CMA1
CSB-MP324745CMA1
CSB-EP324745CMA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype D Protein Tat CSB-YP324746HLJ
CSB-EP324746HLJ
CSB-BP324746HLJ
CSB-MP324746HLJ
CSB-EP324746HLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Relaxosome protein TraM (traM) CSB-YP324747ENL
CSB-EP324747ENL
CSB-BP324747ENL
CSB-MP324747ENL
CSB-EP324747ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter aerogenes Maltose transport system permease protein malG (malG), partial CSB-YP324748EKN1
CSB-EP324748EKN1
CSB-BP324748EKN1
CSB-MP324748EKN1
CSB-EP324748EKN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans NADP-specific glutamate dehydrogenase (gdhA) CSB-YP324749EKF
CSB-EP324749EKF
CSB-BP324749EKF
CSB-MP324749EKF
CSB-EP324749EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuticle collagen 14 (col-14) CSB-YP324750CXY
CSB-EP324750CXY
CSB-BP324750CXY
CSB-MP324750CXY
CSB-EP324750CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Oocyte zinc finger protein XlCOF8.4I CSB-YP324751XBE
CSB-EP324751XBE
CSB-BP324751XBE
CSB-MP324751XBE
CSB-EP324751XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza B virus Hemagglutinin (HA) CSB-YP324752IJI
CSB-EP324752IJI
CSB-BP324752IJI
CSB-MP324752IJI
CSB-EP324752IJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Transcription regulatory protein SNF6 (SNF6) CSB-YP324753SVG
CSB-EP324753SVG
CSB-BP324753SVG
CSB-MP324753SVG
CSB-EP324753SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Stress protein DDR48 (DDR48) CSB-YP324754SVG
CSB-EP324754SVG
CSB-BP324754SVG
CSB-MP324754SVG
CSB-EP324754SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor Tu, chloroplastic (tufA) CSB-YP324755CNB
CSB-EP324755CNB
CSB-BP324755CNB
CSB-MP324755CNB
CSB-EP324755CNB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Flagellin fljL (fljL) CSB-YP324756DQO
CSB-EP324756DQO
CSB-BP324756DQO
CSB-MP324756DQO
CSB-EP324756DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Uncharacterized 5.1 kDa protein in Gp52-ac intergenic region (y16J) CSB-YP324757EDZ
CSB-EP324757EDZ
CSB-BP324757EDZ
CSB-MP324757EDZ
CSB-EP324757EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vitellogenin-6 (vit-6), partial CSB-YP324758CXY
CSB-EP324758CXY
CSB-BP324758CXY
CSB-MP324758CXY
CSB-EP324758CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase YPK2/YKR2 (YPK2), partial CSB-YP324760SVG
CSB-EP324760SVG
CSB-BP324760SVG
CSB-MP324760SVG
CSB-EP324760SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chironomus thummi piger Globin CTT-VIIB-8 (CTT-7B8) CSB-YP324761CIQ
CSB-EP324761CIQ
CSB-BP324761CIQ
CSB-MP324761CIQ
CSB-EP324761CIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uromastyx hardwickii Hemoglobin subunit beta-2 CSB-YP324762UAV
CSB-EP324762UAV
CSB-BP324762UAV
CSB-MP324762UAV
CSB-EP324762UAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca nemestrina Hemoglobin subunit alpha-1/2/3 CSB-YP324763MOX
CSB-EP324763MOX
CSB-BP324763MOX
CSB-MP324763MOX
CSB-EP324763MOX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Vitellogenin-A1 CSB-YP324764XBE
CSB-EP324764XBE
CSB-BP324764XBE
CSB-MP324764XBE
CSB-EP324764XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Feline immunodeficiency virus Envelope glycoprotein gp150 (env), partial CSB-YP324765FAM
CSB-EP324765FAM
CSB-BP324765FAM
CSB-MP324765FAM
CSB-EP324765FAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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