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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mycobacterium gilvum 30S ribosomal protein S20 (rpsT) CSB-YP391882MOK
CSB-EP391882MOK
CSB-BP391882MOK
CSB-MP391882MOK
CSB-EP391882MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Glutamate 5-kinase (proB) CSB-YP391883MOK
CSB-EP391883MOK
CSB-BP391883MOK
CSB-MP391883MOK
CSB-EP391883MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Uncharacterized methyltransferase Mflv_0427 (Mflv_0427) CSB-YP391884MOK
CSB-EP391884MOK
CSB-BP391884MOK
CSB-MP391884MOK
CSB-EP391884MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum UPF0301 protein Mflv_0850 (Mflv_0850) CSB-YP391885MOK
CSB-EP391885MOK
CSB-BP391885MOK
CSB-MP391885MOK
CSB-EP391885MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 50S ribosomal protein L34 (rpmH) CSB-YP391886MOK
CSB-EP391886MOK
CSB-BP391886MOK
CSB-MP391886MOK
CSB-EP391886MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Lysine--tRNA ligase (lysS), partial CSB-YP391887MOK
CSB-EP391887MOK
CSB-BP391887MOK
CSB-MP391887MOK
CSB-EP391887MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Aspartate 1-decarboxylase (panD) CSB-YP391888MOK
CSB-EP391888MOK
CSB-BP391888MOK
CSB-MP391888MOK
CSB-EP391888MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 30S ribosomal protein S18 2 (rpsR2) CSB-YP391889MOK
CSB-EP391889MOK
CSB-BP391889MOK
CSB-MP391889MOK
CSB-EP391889MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Prephenate dehydratase (pheA) CSB-YP391890MOK
CSB-EP391890MOK
CSB-BP391890MOK
CSB-MP391890MOK
CSB-EP391890MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Enolase (eno) CSB-YP391891MOK
CSB-EP391891MOK
CSB-BP391891MOK
CSB-MP391891MOK
CSB-EP391891MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Mycothiol acetyltransferase (mshD) CSB-YP391892MOK
CSB-EP391892MOK
CSB-BP391892MOK
CSB-MP391892MOK
CSB-EP391892MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum UPF0678 fatty acid-binding protein-like protein Mflv_1642 (Mflv_1642) CSB-YP391893MOK
CSB-EP391893MOK
CSB-BP391893MOK
CSB-MP391893MOK
CSB-EP391893MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 3- (3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase, partial CSB-YP391894MOK
CSB-EP391894MOK
CSB-BP391894MOK
CSB-MP391894MOK
CSB-EP391894MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum ATP synthase subunit b-delta (atpFH), partial CSB-YP391895MOK
CSB-EP391895MOK
CSB-BP391895MOK
CSB-MP391895MOK
CSB-EP391895MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum D-tyrosyl-tRNA (Tyr) deacylase CSB-YP391896MOK
CSB-EP391896MOK
CSB-BP391896MOK
CSB-MP391896MOK
CSB-EP391896MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Putative O-methyltransferase Mflv_2199 (Mflv_2199) CSB-YP391897MOK
CSB-EP391897MOK
CSB-BP391897MOK
CSB-MP391897MOK
CSB-EP391897MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP391898MOK
CSB-EP391898MOK
CSB-BP391898MOK
CSB-MP391898MOK
CSB-EP391898MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum UPF0353 protein Mflv_3659 (Mflv_3659), partial CSB-YP391899MOK1
CSB-EP391899MOK1
CSB-BP391899MOK1
CSB-MP391899MOK1
CSB-EP391899MOK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Quinolinate synthase A (nadA) CSB-YP391900MOK
CSB-EP391900MOK
CSB-BP391900MOK
CSB-MP391900MOK
CSB-EP391900MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP391901MOK
CSB-EP391901MOK
CSB-BP391901MOK
CSB-MP391901MOK
CSB-EP391901MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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