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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Yersinia pestis Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP395844YAL
CSB-EP395844YAL
CSB-BP395844YAL
CSB-MP395844YAL
CSB-EP395844YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis DnaA-homolog protein hda (hda) CSB-YP395845YAL
CSB-EP395845YAL
CSB-BP395845YAL
CSB-MP395845YAL
CSB-EP395845YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Putative multidrug resistance protein MdtD (mdtD), partial CSB-YP395846YAL
CSB-EP395846YAL
CSB-BP395846YAL
CSB-MP395846YAL
CSB-EP395846YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Peptidase B (pepB) CSB-YP395847YAL
CSB-EP395847YAL
CSB-BP395847YAL
CSB-MP395847YAL
CSB-EP395847YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Dihydroorotate dehydrogenase (quinone) CSB-YP395848YAL
CSB-EP395848YAL
CSB-BP395848YAL
CSB-MP395848YAL
CSB-EP395848YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis UPF0434 protein YPDSF_2296 (YPDSF_2296) CSB-YP395849YAL
CSB-EP395849YAL
CSB-BP395849YAL
CSB-MP395849YAL
CSB-EP395849YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Deoxyribose-phosphate aldolase (deoC) CSB-YP395850YAL
CSB-EP395850YAL
CSB-BP395850YAL
CSB-MP395850YAL
CSB-EP395850YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP395851YAL
CSB-EP395851YAL
CSB-BP395851YAL
CSB-MP395851YAL
CSB-EP395851YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis SsrA-binding protein (smpB) CSB-YP395852YAL
CSB-EP395852YAL
CSB-BP395852YAL
CSB-MP395852YAL
CSB-EP395852YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP395853YAL
CSB-EP395853YAL
CSB-BP395853YAL
CSB-MP395853YAL
CSB-EP395853YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis 50S ribosomal protein L31 type B (rpmE2) CSB-YP395854YAL
CSB-EP395854YAL
CSB-BP395854YAL
CSB-MP395854YAL
CSB-EP395854YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP395855YAL
CSB-EP395855YAL
CSB-BP395855YAL
CSB-MP395855YAL
CSB-EP395855YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP395856YAL
CSB-EP395856YAL
CSB-BP395856YAL
CSB-MP395856YAL
CSB-EP395856YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis UPF0255 protein YPDSF_2853 (YPDSF_2853) CSB-YP395857YAL
CSB-EP395857YAL
CSB-BP395857YAL
CSB-MP395857YAL
CSB-EP395857YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis GMP reductase (guaC) CSB-YP395858YAL
CSB-EP395858YAL
CSB-BP395858YAL
CSB-MP395858YAL
CSB-EP395858YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Sugar fermentation stimulation protein homolog (sfsA) CSB-YP395859YAL
CSB-EP395859YAL
CSB-BP395859YAL
CSB-MP395859YAL
CSB-EP395859YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Cell division protein FtsB (ftsB), partial CSB-YP395860YAL
CSB-EP395860YAL
CSB-BP395860YAL
CSB-MP395860YAL
CSB-EP395860YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Carbon storage regulator homolog (csrA) CSB-YP395861YAL
CSB-EP395861YAL
CSB-BP395861YAL
CSB-MP395861YAL
CSB-EP395861YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP395862YAL
CSB-EP395862YAL
CSB-BP395862YAL
CSB-MP395862YAL
CSB-EP395862YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Protein ApaG (apaG) CSB-YP395863YAL
CSB-EP395863YAL
CSB-BP395863YAL
CSB-MP395863YAL
CSB-EP395863YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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