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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Acidianus bottle-shaped virus Uncharacterized protein ORF95 (ORF95), partial CSB-YP397466ATY
CSB-EP397466ATY
CSB-BP397466ATY
CSB-MP397466ATY
CSB-EP397466ATY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus bottle-shaped virus Uncharacterized protein ORF59 (ORF59), partial CSB-YP397467ATY
CSB-EP397467ATY
CSB-BP397467ATY
CSB-MP397467ATY
CSB-EP397467ATY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus bottle-shaped virus Uncharacterized protein ORF48b (ORF48b) CSB-YP397468ATY
CSB-EP397468ATY
CSB-BP397468ATY
CSB-MP397468ATY
CSB-EP397468ATY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus bottle-shaped virus Uncharacterized protein OR117 (OR117) CSB-YP397469ATY
CSB-EP397469ATY
CSB-BP397469ATY
CSB-MP397469ATY
CSB-EP397469ATY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein ykfM (ykfM), partial CSB-YP397470ENV1
CSB-EP397470ENV1
CSB-BP397470ENV1
CSB-MP397470ENV1
CSB-EP397470ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yneM (yneM), partial CSB-YP397471ENV
CSB-EP397471ENV
CSB-BP397471ENV
CSB-MP397471ENV
CSB-EP397471ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative uncharacterized protein yibV (yibV) CSB-YP397472ENV
CSB-EP397472ENV
CSB-BP397472ENV
CSB-MP397472ENV
CSB-EP397472ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Protein FAM107A (FAM107A) CSB-YP397473EQV
CSB-EP397473EQV
CSB-BP397473EQV
CSB-MP397473EQV
CSB-EP397473EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acaryochloris marina Photosystem Q (B) protein 2, partial CSB-YP397474AVV1
CSB-EP397474AVV1
CSB-BP397474AVV1
CSB-MP397474AVV1
CSB-EP397474AVV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable rhamnogalacturonate lyase B (rglB), partial CSB-YP397475AVE
CSB-EP397475AVE
CSB-BP397475AVE
CSB-MP397475AVE
CSB-EP397475AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 30S ribosomal protein S21 (rpsU) CSB-YP397476ODJ
CSB-EP397476ODJ
CSB-BP397476ODJ
CSB-MP397476ODJ
CSB-EP397476ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP397477ODJ
CSB-EP397477ODJ
CSB-BP397477ODJ
CSB-MP397477ODJ
CSB-EP397477ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Elongation factor Tu 1 (tuf1) CSB-YP397478ODJ
CSB-EP397478ODJ
CSB-BP397478ODJ
CSB-MP397478ODJ
CSB-EP397478ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP397479ODJ
CSB-EP397479ODJ
CSB-BP397479ODJ
CSB-MP397479ODJ
CSB-EP397479ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 30S ribosomal protein S14 (rpsN) CSB-YP397480ODJ
CSB-EP397480ODJ
CSB-BP397480ODJ
CSB-MP397480ODJ
CSB-EP397480ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 30S ribosomal protein S3 (rpsC) CSB-YP397481ODJ
CSB-EP397481ODJ
CSB-BP397481ODJ
CSB-MP397481ODJ
CSB-EP397481ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Leucine--tRNA ligase (leuS), partial CSB-YP397482ODJ
CSB-EP397482ODJ
CSB-BP397482ODJ
CSB-MP397482ODJ
CSB-EP397482ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 50S ribosomal protein L20 (rplT) CSB-YP397483ODJ
CSB-EP397483ODJ
CSB-BP397483ODJ
CSB-MP397483ODJ
CSB-EP397483ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP397484ODJ
CSB-EP397484ODJ
CSB-BP397484ODJ
CSB-MP397484ODJ
CSB-EP397484ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi DNA mismatch repair protein MutS (mutS), partial CSB-YP397485ODJ
CSB-EP397485ODJ
CSB-BP397485ODJ
CSB-MP397485ODJ
CSB-EP397485ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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