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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Orientia tsutsugamushi Elongation factor 4 (lepA), partial CSB-YP397486ODJ
CSB-EP397486ODJ
CSB-BP397486ODJ
CSB-MP397486ODJ
CSB-EP397486ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP397487ODJ
CSB-EP397487ODJ
CSB-BP397487ODJ
CSB-MP397487ODJ
CSB-EP397487ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Ribonuclease H (rnhA) CSB-YP397488ODJ
CSB-EP397488ODJ
CSB-BP397488ODJ
CSB-MP397488ODJ
CSB-EP397488ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Cysteine--tRNA ligase (cysS) CSB-YP397489ODJ
CSB-EP397489ODJ
CSB-BP397489ODJ
CSB-MP397489ODJ
CSB-EP397489ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis DNA replication and repair protein recF (recF) CSB-YP397490CWT
CSB-EP397490CWT
CSB-BP397490CWT
CSB-MP397490CWT
CSB-EP397490CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Uracil phosphoribosyltransferase (upp) CSB-YP397491CWT
CSB-EP397491CWT
CSB-BP397491CWT
CSB-MP397491CWT
CSB-EP397491CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP397492CWT
CSB-EP397492CWT
CSB-BP397492CWT
CSB-MP397492CWT
CSB-EP397492CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis UPF0182 protein CMM_1204 (CMM_1204), partial CSB-YP397493CWT
CSB-EP397493CWT
CSB-BP397493CWT
CSB-MP397493CWT
CSB-EP397493CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Homoserine O-acetyltransferase (metX) CSB-YP397494CWT
CSB-EP397494CWT
CSB-BP397494CWT
CSB-MP397494CWT
CSB-EP397494CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis tRNA (guanine-N (1)-)-methyltransferase CSB-YP397495CWT
CSB-EP397495CWT
CSB-BP397495CWT
CSB-MP397495CWT
CSB-EP397495CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP397496CWT
CSB-EP397496CWT
CSB-BP397496CWT
CSB-MP397496CWT
CSB-EP397496CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis UPF0042 nucleotide-binding protein CMM_1747 (CMM_1747) CSB-YP397497CWT
CSB-EP397497CWT
CSB-BP397497CWT
CSB-MP397497CWT
CSB-EP397497CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis S-adenosylmethionine synthase (metK) CSB-YP397498CWT
CSB-EP397498CWT
CSB-BP397498CWT
CSB-MP397498CWT
CSB-EP397498CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Elongation factor P (efp) CSB-YP397499CWT
CSB-EP397499CWT
CSB-BP397499CWT
CSB-MP397499CWT
CSB-EP397499CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Putative Holliday junction resolvase (CMM_1801) CSB-YP397500CWT
CSB-EP397500CWT
CSB-BP397500CWT
CSB-MP397500CWT
CSB-EP397500CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Dihydroorotate dehydrogenase (quinone) CSB-YP397501CWT
CSB-EP397501CWT
CSB-BP397501CWT
CSB-MP397501CWT
CSB-EP397501CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP397502CWT
CSB-EP397502CWT
CSB-BP397502CWT
CSB-MP397502CWT
CSB-EP397502CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP397503CWT
CSB-EP397503CWT
CSB-BP397503CWT
CSB-MP397503CWT
CSB-EP397503CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis 50S ribosomal protein L20 (rplT) CSB-YP397504CWT
CSB-EP397504CWT
CSB-BP397504CWT
CSB-MP397504CWT
CSB-EP397504CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Enolase (eno) CSB-YP397505CWT
CSB-EP397505CWT
CSB-BP397505CWT
CSB-MP397505CWT
CSB-EP397505CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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