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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Solanum tuberosum Calcium-dependent protein kinase 4 (CPK4), partial CSB-YP397686FIG
CSB-EP397686FIG
CSB-BP397686FIG
CSB-MP397686FIG
CSB-EP397686FIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger ATP-dependent RNA helicase fal1 (fal1) CSB-YP397687AVE
CSB-EP397687AVE
CSB-BP397687AVE
CSB-MP397687AVE
CSB-EP397687AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Pro-apoptotic serine protease nma111 (nma111), partial CSB-YP397688AVE
CSB-EP397688AVE
CSB-BP397688AVE
CSB-MP397688AVE
CSB-EP397688AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable beta-glucosidase M (bglM), partial CSB-YP397689AVE
CSB-EP397689AVE
CSB-BP397689AVE
CSB-MP397689AVE
CSB-EP397689AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Ubiquinone biosynthesis protein coq4, mitochondrial (coq4) CSB-YP397690AVE
CSB-EP397690AVE
CSB-BP397690AVE
CSB-MP397690AVE
CSB-EP397690AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vitis vinifera Arginine biosynthesis bifunctional protein ArgJ, chloroplastic (VITISV_037692), partial CSB-YP397691VFQ
CSB-EP397691VFQ
CSB-BP397691VFQ
CSB-MP397691VFQ
CSB-EP397691VFQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vitis vinifera Lipoyl synthase, mitochondrial (LIP1), partial CSB-YP397692VFQ
CSB-EP397692VFQ
CSB-BP397692VFQ
CSB-MP397692VFQ
CSB-EP397692VFQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP397693ODJ
CSB-EP397693ODJ
CSB-BP397693ODJ
CSB-MP397693ODJ
CSB-EP397693ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Recombination protein RecR (recR) CSB-YP397694ODJ
CSB-EP397694ODJ
CSB-BP397694ODJ
CSB-MP397694ODJ
CSB-EP397694ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Valine--tRNA ligase (valS), partial CSB-YP397695ODJ
CSB-EP397695ODJ
CSB-BP397695ODJ
CSB-MP397695ODJ
CSB-EP397695ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 30S ribosomal protein S13 (rpsM) CSB-YP397696ODJ
CSB-EP397696ODJ
CSB-BP397696ODJ
CSB-MP397696ODJ
CSB-EP397696ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 50S ribosomal protein L24 (rplX) CSB-YP397697ODJ
CSB-EP397697ODJ
CSB-BP397697ODJ
CSB-MP397697ODJ
CSB-EP397697ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 50S ribosomal protein L22 (rplV) CSB-YP397698ODJ
CSB-EP397698ODJ
CSB-BP397698ODJ
CSB-MP397698ODJ
CSB-EP397698ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 50S ribosomal protein L9 (rplI) CSB-YP397699ODJ
CSB-EP397699ODJ
CSB-BP397699ODJ
CSB-MP397699ODJ
CSB-EP397699ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 50S ribosomal protein L35 (rpmI) CSB-YP397700ODJ
CSB-EP397700ODJ
CSB-BP397700ODJ
CSB-MP397700ODJ
CSB-EP397700ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Protein translocase subunit SecA (secA), partial CSB-YP397701ODJ
CSB-EP397701ODJ
CSB-BP397701ODJ
CSB-MP397701ODJ
CSB-EP397701ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP397702ODJ
CSB-EP397702ODJ
CSB-BP397702ODJ
CSB-MP397702ODJ
CSB-EP397702ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 60 kDa chaperonin (groL), partial CSB-YP397703ODJ
CSB-EP397703ODJ
CSB-BP397703ODJ
CSB-MP397703ODJ
CSB-EP397703ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Elongation factor P (efp) CSB-YP397704ODJ
CSB-EP397704ODJ
CSB-BP397704ODJ
CSB-MP397704ODJ
CSB-EP397704ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP397705ODJ
CSB-EP397705ODJ
CSB-BP397705ODJ
CSB-MP397705ODJ
CSB-EP397705ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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