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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Actinobacillus pleuropneumoniae serotype 7 Serine--tRNA ligase (serS) CSB-YP455339AXI
CSB-EP455339AXI
CSB-BP455339AXI
CSB-MP455339AXI
CSB-EP455339AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Deoxyribose-phosphate aldolase (deoC) CSB-YP455340AXI
CSB-EP455340AXI
CSB-BP455340AXI
CSB-MP455340AXI
CSB-EP455340AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Phosphoglycerate kinase (pgk) CSB-YP455341AXI
CSB-EP455341AXI
CSB-BP455341AXI
CSB-MP455341AXI
CSB-EP455341AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 DNA ligase (ligA), partial CSB-YP455342AXI
CSB-EP455342AXI
CSB-BP455342AXI
CSB-MP455342AXI
CSB-EP455342AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Membrane protein insertase YidC (yidC), partial CSB-YP455343AXI1
CSB-EP455343AXI1
CSB-BP455343AXI1
CSB-MP455343AXI1
CSB-EP455343AXI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 DNA mismatch repair protein MutS (mutS), partial CSB-YP455344AXI
CSB-EP455344AXI
CSB-BP455344AXI
CSB-MP455344AXI
CSB-EP455344AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 ATP synthase gamma chain (atpG) CSB-YP455345AXI
CSB-EP455345AXI
CSB-BP455345AXI
CSB-MP455345AXI
CSB-EP455345AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP455346AXI
CSB-EP455346AXI
CSB-BP455346AXI
CSB-MP455346AXI
CSB-EP455346AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 tRNA1 (Val) (adenine (37)-N6)-methyltransferase CSB-YP455347AXI
CSB-EP455347AXI
CSB-BP455347AXI
CSB-MP455347AXI
CSB-EP455347AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Gamma-glutamyl phosphate reductase (proA) CSB-YP455348AXI
CSB-EP455348AXI
CSB-BP455348AXI
CSB-MP455348AXI
CSB-EP455348AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 UPF0758 protein APP7_2058 (APP7_2058) CSB-YP455349AXI
CSB-EP455349AXI
CSB-BP455349AXI
CSB-MP455349AXI
CSB-EP455349AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At5g66631 (At5g66631), partial CSB-YP455350DOA
CSB-EP455350DOA
CSB-BP455350DOA
CSB-MP455350DOA
CSB-EP455350DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable peroxygenase 7 (PXG7) CSB-YP455351DOA
CSB-EP455351DOA
CSB-BP455351DOA
CSB-MP455351DOA
CSB-EP455351DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Serine/arginine repetitive matrix protein 5 (SRRM5) CSB-YP455352HU
CSB-EP455352HU
CSB-BP455352HU
CSB-MP455352HU
CSB-EP455352HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium knowlesi Anamorsin homolog (PKH_050640) CSB-YP455353EWU
CSB-EP455353EWU
CSB-BP455353EWU
CSB-MP455353EWU
CSB-EP455353EWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium knowlesi tRNA (guanine (37)-N1)-methyltransferase, partial CSB-YP455354EWU
CSB-EP455354EWU
CSB-BP455354EWU
CSB-MP455354EWU
CSB-EP455354EWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium knowlesi Adenylosuccinate synthetase (PKH_111670) CSB-YP455355EWU
CSB-EP455355EWU
CSB-BP455355EWU
CSB-MP455355EWU
CSB-EP455355EWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ubiquinone biosynthesis protein COQ4, mitochondrial (COQ4) CSB-YP455356SVP
CSB-EP455356SVP
CSB-BP455356SVP
CSB-MP455356SVP
CSB-EP455356SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Pentafunctional AROM polypeptide (ARO1), partial CSB-YP455357SVP
CSB-EP455357SVP
CSB-BP455357SVP
CSB-MP455357SVP
CSB-EP455357SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Mitochondrial distribution and morphology protein 34 (MDM34) CSB-YP455358SVP
CSB-EP455358SVP
CSB-BP455358SVP
CSB-MP455358SVP
CSB-EP455358SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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