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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Nostoc punctiforme Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP454478NHQ
CSB-EP454478NHQ
CSB-BP454478NHQ
CSB-MP454478NHQ
CSB-EP454478NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP454479NHQ
CSB-EP454479NHQ
CSB-BP454479NHQ
CSB-MP454479NHQ
CSB-EP454479NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Probable 30S ribosomal protein PSRP-3 (Npun_F4413) CSB-YP454480NHQ
CSB-EP454480NHQ
CSB-BP454480NHQ
CSB-MP454480NHQ
CSB-EP454480NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme RNA 3'-terminal phosphate cyclase (rtcA) CSB-YP454481NHQ
CSB-EP454481NHQ
CSB-BP454481NHQ
CSB-MP454481NHQ
CSB-EP454481NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP454482NHQ
CSB-EP454482NHQ
CSB-BP454482NHQ
CSB-MP454482NHQ
CSB-EP454482NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP454483NHQ
CSB-EP454483NHQ
CSB-BP454483NHQ
CSB-MP454483NHQ
CSB-EP454483NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme 50S ribosomal protein L20 (rplT) CSB-YP454484NHQ
CSB-EP454484NHQ
CSB-BP454484NHQ
CSB-MP454484NHQ
CSB-EP454484NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme 33 kDa chaperonin (hslO) CSB-YP454485NHQ
CSB-EP454485NHQ
CSB-BP454485NHQ
CSB-MP454485NHQ
CSB-EP454485NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme GTPase Der (der) CSB-YP454486NHQ
CSB-EP454486NHQ
CSB-BP454486NHQ
CSB-MP454486NHQ
CSB-EP454486NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP454487NHQ
CSB-EP454487NHQ
CSB-BP454487NHQ
CSB-MP454487NHQ
CSB-EP454487NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Phosphomethylpyrimidine synthase (thiC) CSB-YP454488NHQ
CSB-EP454488NHQ
CSB-BP454488NHQ
CSB-MP454488NHQ
CSB-EP454488NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme N utilization substance protein B homolog (nusB) CSB-YP454489NHQ
CSB-EP454489NHQ
CSB-BP454489NHQ
CSB-MP454489NHQ
CSB-EP454489NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Photosystem I P700 chlorophyll a apoprotein A1 (psaA), partial CSB-YP454490NHQ
CSB-EP454490NHQ
CSB-BP454490NHQ
CSB-MP454490NHQ
CSB-EP454490NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme 30S ribosomal protein S12 (rpsL) CSB-YP454491NHQ
CSB-EP454491NHQ
CSB-BP454491NHQ
CSB-MP454491NHQ
CSB-EP454491NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP454492NHQ
CSB-EP454492NHQ
CSB-BP454492NHQ
CSB-MP454492NHQ
CSB-EP454492NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP454493NHQ
CSB-EP454493NHQ
CSB-BP454493NHQ
CSB-MP454493NHQ
CSB-EP454493NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme 30S ribosomal protein S14 (rpsN) CSB-YP454494NHQ
CSB-EP454494NHQ
CSB-BP454494NHQ
CSB-MP454494NHQ
CSB-EP454494NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme 30S ribosomal protein S2 (rpsB) CSB-YP454495NHQ
CSB-EP454495NHQ
CSB-BP454495NHQ
CSB-MP454495NHQ
CSB-EP454495NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme NAD (P)H-quinone oxidoreductase subunit 1, partial CSB-YP454496NHQ
CSB-EP454496NHQ
CSB-BP454496NHQ
CSB-MP454496NHQ
CSB-EP454496NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP454497NHQ
CSB-EP454497NHQ
CSB-BP454497NHQ
CSB-MP454497NHQ
CSB-EP454497NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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