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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Treponema pallidum subsp. pallidum Threonine--tRNA ligase (thrS), partial CSB-YP454678TPS
CSB-EP454678TPS
CSB-BP454678TPS
CSB-MP454678TPS
CSB-EP454678TPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum subsp. pallidum Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP454679TPS
CSB-EP454679TPS
CSB-BP454679TPS
CSB-MP454679TPS
CSB-EP454679TPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum subsp. pallidum 50S ribosomal protein L19 (rplS) CSB-YP454680TPS
CSB-EP454680TPS
CSB-BP454680TPS
CSB-MP454680TPS
CSB-EP454680TPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum subsp. pallidum Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP454681TPS
CSB-EP454681TPS
CSB-BP454681TPS
CSB-MP454681TPS
CSB-EP454681TPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum subsp. pallidum Regulatory protein recX (recX) CSB-YP454682TPS
CSB-EP454682TPS
CSB-BP454682TPS
CSB-MP454682TPS
CSB-EP454682TPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP454683BWR
CSB-EP454683BWR
CSB-BP454683BWR
CSB-MP454683BWR
CSB-EP454683BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus N utilization substance protein B homolog (nusB) CSB-YP454684BWR
CSB-EP454684BWR
CSB-BP454684BWR
CSB-MP454684BWR
CSB-EP454684BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Heme A synthase (ctaA), partial CSB-YP454685BWR
CSB-EP454685BWR
CSB-BP454685BWR
CSB-MP454685BWR
CSB-EP454685BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP454686BWR
CSB-EP454686BWR
CSB-BP454686BWR
CSB-MP454686BWR
CSB-EP454686BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Sec-independent protein translocase protein TatA (tatA), partial CSB-YP454687BWR
CSB-EP454687BWR
CSB-BP454687BWR
CSB-MP454687BWR
CSB-EP454687BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP454688BWR
CSB-EP454688BWR
CSB-BP454688BWR
CSB-MP454688BWR
CSB-EP454688BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Flavoprotein wrbA (wrbA) CSB-YP454689BWR
CSB-EP454689BWR
CSB-BP454689BWR
CSB-MP454689BWR
CSB-EP454689BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Protein hfq (hfq) CSB-YP454690BWR
CSB-EP454690BWR
CSB-BP454690BWR
CSB-MP454690BWR
CSB-EP454690BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP454691BWR
CSB-EP454691BWR
CSB-BP454691BWR
CSB-MP454691BWR
CSB-EP454691BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP454692BWR
CSB-EP454692BWR
CSB-BP454692BWR
CSB-MP454692BWR
CSB-EP454692BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 30S ribosomal protein S11 (rpsK) CSB-YP454693BWR
CSB-EP454693BWR
CSB-BP454693BWR
CSB-MP454693BWR
CSB-EP454693BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 50S ribosomal protein L29 (rpmC) CSB-YP454694BWR
CSB-EP454694BWR
CSB-BP454694BWR
CSB-MP454694BWR
CSB-EP454694BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 50S ribosomal protein L4 (rplD) CSB-YP454695BWR
CSB-EP454695BWR
CSB-BP454695BWR
CSB-MP454695BWR
CSB-EP454695BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP454696BWR
CSB-EP454696BWR
CSB-BP454696BWR
CSB-MP454696BWR
CSB-EP454696BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 50S ribosomal protein L11 (rplK) CSB-YP454697BWR
CSB-EP454697BWR
CSB-BP454697BWR
CSB-MP454697BWR
CSB-EP454697BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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