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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Erwinia tasmaniensis 50S ribosomal protein L33 (rpmG) CSB-YP455078ENB
CSB-EP455078ENB
CSB-BP455078ENB
CSB-MP455078ENB
CSB-EP455078ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Der GTPase-activating protein YihI (yihI) CSB-YP455079ENB
CSB-EP455079ENB
CSB-BP455079ENB
CSB-MP455079ENB
CSB-EP455079ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis ATP phosphoribosyltransferase (hisG) CSB-YP455080ENB
CSB-EP455080ENB
CSB-BP455080ENB
CSB-MP455080ENB
CSB-EP455080ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP455081ENB
CSB-EP455081ENB
CSB-BP455081ENB
CSB-MP455081ENB
CSB-EP455081ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP455082ENB
CSB-EP455082ENB
CSB-BP455082ENB
CSB-MP455082ENB
CSB-EP455082ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP455083ENB
CSB-EP455083ENB
CSB-BP455083ENB
CSB-MP455083ENB
CSB-EP455083ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Putative ECA polymerase (wzyE), partial CSB-YP455084ENB
CSB-EP455084ENB
CSB-BP455084ENB
CSB-MP455084ENB
CSB-EP455084ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Pantothenate kinase (coaA) CSB-YP455085ENB
CSB-EP455085ENB
CSB-BP455085ENB
CSB-MP455085ENB
CSB-EP455085ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP455086ENB
CSB-EP455086ENB
CSB-BP455086ENB
CSB-MP455086ENB
CSB-EP455086ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis tRNA (uracil (54)-C (5))-methyltransferase CSB-YP455087ENB
CSB-EP455087ENB
CSB-BP455087ENB
CSB-MP455087ENB
CSB-EP455087ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) CSB-YP455088ENB
CSB-EP455088ENB
CSB-BP455088ENB
CSB-MP455088ENB
CSB-EP455088ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis UPF0246 protein ETA_07010 (ETA_07010) CSB-YP455089ENB
CSB-EP455089ENB
CSB-BP455089ENB
CSB-MP455089ENB
CSB-EP455089ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis GTPase obg (obg) CSB-YP455090ENB
CSB-EP455090ENB
CSB-BP455090ENB
CSB-MP455090ENB
CSB-EP455090ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis UPF0042 nucleotide-binding protein ETA_03120 (ETA_03120) CSB-YP455091ENB
CSB-EP455091ENB
CSB-BP455091ENB
CSB-MP455091ENB
CSB-EP455091ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Lipoprotein signal peptidase (lspA), partial CSB-YP455092ENB1
CSB-EP455092ENB1
CSB-BP455092ENB1
CSB-MP455092ENB1
CSB-EP455092ENB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Dihydrodipicolinate reductase (dapB) CSB-YP455093ENB
CSB-EP455093ENB
CSB-BP455093ENB
CSB-MP455093ENB
CSB-EP455093ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP455094ENB
CSB-EP455094ENB
CSB-BP455094ENB
CSB-MP455094ENB
CSB-EP455094ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis 30S ribosomal protein S16 (rpsP) CSB-YP455095ENB
CSB-EP455095ENB
CSB-BP455095ENB
CSB-MP455095ENB
CSB-EP455095ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP455096ENB
CSB-EP455096ENB
CSB-BP455096ENB
CSB-MP455096ENB
CSB-EP455096ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP455097ENB
CSB-EP455097ENB
CSB-BP455097ENB
CSB-MP455097ENB
CSB-EP455097ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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