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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Erwinia tasmaniensis Glutamine--tRNA ligase (glnS), partial CSB-YP455058ENB
CSB-EP455058ENB
CSB-BP455058ENB
CSB-MP455058ENB
CSB-EP455058ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Outer-membrane lipoprotein carrier protein (lolA) CSB-YP455059ENB
CSB-EP455059ENB
CSB-BP455059ENB
CSB-MP455059ENB
CSB-EP455059ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP455060ENB
CSB-EP455060ENB
CSB-BP455060ENB
CSB-MP455060ENB
CSB-EP455060ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis tRNA modification GTPase MnmE (mnmE) CSB-YP455061ENB
CSB-EP455061ENB
CSB-BP455061ENB
CSB-MP455061ENB
CSB-EP455061ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis DNA mismatch repair protein mutL (mutL), partial CSB-YP455062ENB
CSB-EP455062ENB
CSB-BP455062ENB
CSB-MP455062ENB
CSB-EP455062ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Protein hfq (hfq) CSB-YP455063ENB
CSB-EP455063ENB
CSB-BP455063ENB
CSB-MP455063ENB
CSB-EP455063ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Pantothenate synthetase (panC) CSB-YP455064ENB
CSB-EP455064ENB
CSB-BP455064ENB
CSB-MP455064ENB
CSB-EP455064ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP455065ENB
CSB-EP455065ENB
CSB-BP455065ENB
CSB-MP455065ENB
CSB-EP455065ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP455066ENB
CSB-EP455066ENB
CSB-BP455066ENB
CSB-MP455066ENB
CSB-EP455066ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Flagellar transcriptional regulator FlhC (flhC) CSB-YP455067ENB
CSB-EP455067ENB
CSB-BP455067ENB
CSB-MP455067ENB
CSB-EP455067ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis 30S ribosomal protein S2 (rpsB) CSB-YP455068ENB
CSB-EP455068ENB
CSB-BP455068ENB
CSB-MP455068ENB
CSB-EP455068ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Cell division protein ZipA homolog (zipA), partial CSB-YP455069ENB
CSB-EP455069ENB
CSB-BP455069ENB
CSB-MP455069ENB
CSB-EP455069ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Cytoskeleton protein rodZ (rodZ), partial CSB-YP455070ENB
CSB-EP455070ENB
CSB-BP455070ENB
CSB-MP455070ENB
CSB-EP455070ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP455071ENB
CSB-EP455071ENB
CSB-BP455071ENB
CSB-MP455071ENB
CSB-EP455071ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Glutamyl-tRNA reductase (hemA) CSB-YP455072ENB
CSB-EP455072ENB
CSB-BP455072ENB
CSB-MP455072ENB
CSB-EP455072ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis NH (3)-dependent NAD (+) synthetase CSB-YP455073ENB
CSB-EP455073ENB
CSB-BP455073ENB
CSB-MP455073ENB
CSB-EP455073ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis UPF0061 protein ETA_18310 (ETA_18310) CSB-YP455074ENB
CSB-EP455074ENB
CSB-BP455074ENB
CSB-MP455074ENB
CSB-EP455074ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Threonine--tRNA ligase (thrS), partial CSB-YP455075ENB
CSB-EP455075ENB
CSB-BP455075ENB
CSB-MP455075ENB
CSB-EP455075ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Cell division protein ZapB (zapB) CSB-YP455076ENB
CSB-EP455076ENB
CSB-BP455076ENB
CSB-MP455076ENB
CSB-EP455076ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis S-adenosylmethionine synthase (metK) CSB-YP455077ENB
CSB-EP455077ENB
CSB-BP455077ENB
CSB-MP455077ENB
CSB-EP455077ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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