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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Beijerinckia indica subsp. indica 50S ribosomal protein L24 (rplX) CSB-YP462182BSP
CSB-EP462182BSP
CSB-BP462182BSP
CSB-MP462182BSP
CSB-EP462182BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica 50S ribosomal protein L30 (rpmD) CSB-YP462183BSP
CSB-EP462183BSP
CSB-BP462183BSP
CSB-MP462183BSP
CSB-EP462183BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Anthranilate phosphoribosyltransferase (trpD) CSB-YP462184BSP
CSB-EP462184BSP
CSB-BP462184BSP
CSB-MP462184BSP
CSB-EP462184BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica NADH-quinone oxidoreductase subunit D 2 (nuoD2) CSB-YP462185BSP
CSB-EP462185BSP
CSB-BP462185BSP
CSB-MP462185BSP
CSB-EP462185BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae MutS2 protein (mutS2), partial CSB-YP462186FMZ
CSB-EP462186FMZ
CSB-BP462186FMZ
CSB-MP462186FMZ
CSB-EP462186FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP462187FMZ
CSB-EP462187FMZ
CSB-BP462187FMZ
CSB-MP462187FMZ
CSB-EP462187FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Regulatory protein recX (recX) CSB-YP462188FMZ
CSB-EP462188FMZ
CSB-BP462188FMZ
CSB-MP462188FMZ
CSB-EP462188FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Chaperone protein DnaK (dnaK), partial CSB-YP462189FMZ
CSB-EP462189FMZ
CSB-BP462189FMZ
CSB-MP462189FMZ
CSB-EP462189FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae 50S ribosomal protein L1 (rplA) CSB-YP462190FMZ
CSB-EP462190FMZ
CSB-BP462190FMZ
CSB-MP462190FMZ
CSB-EP462190FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP462191FMZ
CSB-EP462191FMZ
CSB-BP462191FMZ
CSB-MP462191FMZ
CSB-EP462191FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae 50S ribosomal protein L32 (rpmF) CSB-YP462192FMZ
CSB-EP462192FMZ
CSB-BP462192FMZ
CSB-MP462192FMZ
CSB-EP462192FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae tRNA (guanine-N (1)-)-methyltransferase CSB-YP462193FMZ
CSB-EP462193FMZ
CSB-BP462193FMZ
CSB-MP462193FMZ
CSB-EP462193FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae 33 kDa chaperonin (hslO) CSB-YP462194FMZ
CSB-EP462194FMZ
CSB-BP462194FMZ
CSB-MP462194FMZ
CSB-EP462194FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP462195FMZ1
CSB-EP462195FMZ1
CSB-BP462195FMZ1
CSB-MP462195FMZ1
CSB-EP462195FMZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae UPF0346 protein SPCG_0921 (SPCG_0921) CSB-YP462196FMZ
CSB-EP462196FMZ
CSB-BP462196FMZ
CSB-MP462196FMZ
CSB-EP462196FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae SsrA-binding protein (smpB) CSB-YP462197FMZ
CSB-EP462197FMZ
CSB-BP462197FMZ
CSB-MP462197FMZ
CSB-EP462197FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae UvrABC system protein B (uvrB), partial CSB-YP462198FMZ
CSB-EP462198FMZ
CSB-BP462198FMZ
CSB-MP462198FMZ
CSB-EP462198FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae UPF0133 protein SPCG_1178 (SPCG_1178) CSB-YP462199FMZ
CSB-EP462199FMZ
CSB-BP462199FMZ
CSB-MP462199FMZ
CSB-EP462199FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Bifunctional protein pyrR (pyrR) CSB-YP462200FMZ
CSB-EP462200FMZ
CSB-BP462200FMZ
CSB-MP462200FMZ
CSB-EP462200FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Chorismate synthase (aroC) CSB-YP462201FMZ
CSB-EP462201FMZ
CSB-BP462201FMZ
CSB-MP462201FMZ
CSB-EP462201FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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