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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Aliivibrio salmonicida Biotin synthase (bioB) CSB-YP475786AZM
CSB-EP475786AZM
CSB-BP475786AZM
CSB-MP475786AZM
CSB-EP475786AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Probable beta-galactosidase B (lacB), partial CSB-YP475787ETH
CSB-EP475787ETH
CSB-BP475787ETH
CSB-MP475787ETH
CSB-EP475787ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Probable E3 ubiquitin ligase complex SCF subunit sconB (sconB), partial CSB-YP475788ETH
CSB-EP475788ETH
CSB-BP475788ETH
CSB-MP475788ETH
CSB-EP475788ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum 40S ribosomal protein S0 (rps0) CSB-YP475789ETH
CSB-EP475789ETH
CSB-BP475789ETH
CSB-MP475789ETH
CSB-EP475789ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum ATPase get3 (get3) CSB-YP475790ETH
CSB-EP475790ETH
CSB-BP475790ETH
CSB-MP475790ETH
CSB-EP475790ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Pheromone-processing carboxypeptidase kex1 (kex1), partial CSB-YP475791ETH
CSB-EP475791ETH
CSB-BP475791ETH
CSB-MP475791ETH
CSB-EP475791ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Probable cutinase 1 (Pc21g21220) CSB-YP475792ETH
CSB-EP475792ETH
CSB-BP475792ETH
CSB-MP475792ETH
CSB-EP475792ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Mitochondrial distribution and morphology protein 34 1 (mdm34-1), partial CSB-YP475793ETH
CSB-EP475793ETH
CSB-BP475793ETH
CSB-MP475793ETH
CSB-EP475793ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Mannitol-1-phosphate 5-dehydrogenase (Pc22g20800) CSB-YP475794ETH
CSB-EP475794ETH
CSB-BP475794ETH
CSB-MP475794ETH
CSB-EP475794ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable rRNA maturation factor YbeY (ybeY) CSB-YP475795ENW
CSB-EP475795ENW
CSB-BP475795ENW
CSB-MP475795ENW
CSB-EP475795ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Potassium-transporting ATPase A chain (kdpA), partial CSB-YP475796ENW
CSB-EP475796ENW
CSB-BP475796ENW
CSB-MP475796ENW
CSB-EP475796ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Hydroxyethylthiazole kinase (thiM) CSB-YP475797ENW
CSB-EP475797ENW
CSB-BP475797ENW
CSB-MP475797ENW
CSB-EP475797ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli L-carnitine/gamma-butyrobetaine antiporter (caiT), partial CSB-YP475798ENW
CSB-EP475798ENW
CSB-BP475798ENW
CSB-MP475798ENW
CSB-EP475798ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0246 protein yaaA (yaaA) CSB-YP475799ENW
CSB-EP475799ENW
CSB-BP475799ENW
CSB-MP475799ENW
CSB-EP475799ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Bis (5'-nucleosyl)-tetraphosphatase, symmetrical CSB-YP475800ENW
CSB-EP475800ENW
CSB-BP475800ENW
CSB-MP475800ENW
CSB-EP475800ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli RNA polymerase-associated protein RapA (rapA), partial CSB-YP475801ENW
CSB-EP475801ENW
CSB-BP475801ENW
CSB-MP475801ENW
CSB-EP475801ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP475802ENW
CSB-EP475802ENW
CSB-BP475802ENW
CSB-MP475802ENW
CSB-EP475802ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0289 protein yacF (yacF) CSB-YP475803ENW
CSB-EP475803ENW
CSB-BP475803ENW
CSB-MP475803ENW
CSB-EP475803ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Sugar fermentation stimulation protein A (sfsA) CSB-YP475804ENW
CSB-EP475804ENW
CSB-BP475804ENW
CSB-MP475804ENW
CSB-EP475804ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0325 protein yaeH (yaeH) CSB-YP475805ENW
CSB-EP475805ENW
CSB-BP475805ENW
CSB-MP475805ENW
CSB-EP475805ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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