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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae Transaldolase (TAL1) CSB-YP023112SVG
CSB-EP023112SVG
CSB-BP023112SVG
CSB-MP023112SVG
CSB-EP023112SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylococcus capsulatus Glutamine synthetase (glnA) CSB-YP009553MFM
CSB-EP009553MFM
CSB-BP009553MFM
CSB-MP009553MFM
CSB-EP009553MFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii Cytochrome c (CYC1) CSB-YP006328DSJ
CSB-EP006328DSJ
CSB-BP006328DSJ
CSB-MP006328DSJ
CSB-EP006328DSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histone H3.3 CSB-YP010109TLT
CSB-EP010109TLT
CSB-BP010109TLT
CSB-MP010109TLT
CSB-EP010109TLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Strongylocentrotus purpuratus Cytochrome c oxidase subunit 2 (COII), partial CSB-YP015073FOT1
CSB-EP015073FOT1
CSB-BP015073FOT1
CSB-MP015073FOT1
CSB-EP015073FOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Strongylocentrotus purpuratus NADH-ubiquinone oxidoreductase chain 4 (ND4), partial CSB-YP015079FOT
CSB-EP015079FOT
CSB-BP015079FOT
CSB-MP015079FOT
CSB-EP015079FOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Podospora anserina NADH-ubiquinone oxidoreductase chain 4 (ND4), partial CSB-YP015079EXJ
CSB-EP015079EXJ
CSB-BP015079EXJ
CSB-MP015079EXJ
CSB-EP015079EXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histone H1.2 (hil-2) CSB-YP010378CXY
CSB-EP010378CXY
CSB-BP010378CXY
CSB-MP010378CXY
CSB-EP010378CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Histone H1.3 CSB-YP010379TQN
CSB-EP010379TQN
CSB-BP010379TQN
CSB-MP010379TQN
CSB-EP010379TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gadus morhua NADH-ubiquinone oxidoreductase chain 3 (MT-ND3), partial CSB-YP015078GER1
CSB-EP015078GER1
CSB-BP015078GER1
CSB-MP015078GER1
CSB-EP015078GER1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Isocitrate dehydrogenase [NADP] (icd), partial CSB-YP010990DPX
CSB-EP010990DPX
CSB-BP010990DPX
CSB-MP010990DPX
CSB-EP010990DPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae V-type proton ATPase subunit B (VMA2) CSB-YP002398SVG
CSB-EP002398SVG
CSB-BP002398SVG
CSB-MP002398SVG
CSB-EP002398SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dipodomys heermanni Cytochrome b (MT-CYB), partial CSB-YP015075DEQ1
CSB-EP015075DEQ1
CSB-BP015075DEQ1
CSB-MP015075DEQ1
CSB-EP015075DEQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Liophis miliaris Hemoglobin subunit beta (HBB) CSB-YP010150LGM
CSB-EP010150LGM
CSB-BP010150LGM
CSB-MP010150LGM
CSB-EP010150LGM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lithobates catesbeiana NADH-ubiquinone oxidoreductase chain 2 (MT-ND2), partial CSB-YP015077LQA
CSB-EP015077LQA
CSB-BP015077LQA
CSB-MP015077LQA
CSB-EP015077LQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus Geranylgeranyl pyrophosphate synthase (crtE) CSB-YP009393RLD
CSB-EP009393RLD
CSB-BP009393RLD
CSB-MP009393RLD
CSB-EP009393RLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster U1 small nuclear ribonucleoprotein 70 kDa (snRNP-U1-70K) CSB-YP022325DLU
CSB-EP022325DLU
CSB-BP022325DLU
CSB-MP022325DLU
CSB-EP022325DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Transforming growth factor beta-2 (tgfb2) CSB-YP023448XBE
CSB-EP023448XBE
CSB-BP023448XBE
CSB-MP023448XBE
CSB-EP023448XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Transcription factor jun-D (JUND) CSB-YP011974HU
CSB-EP011974HU
CSB-BP011974HU
CSB-MP011974HU
CSB-EP011974HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus borealis Transcription factor IIIA (gtf3a) CSB-YP010021XAO
CSB-EP010021XAO
CSB-BP010021XAO
CSB-MP010021XAO
CSB-EP010021XAO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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