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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Saccharomyces cerevisiae Cytoplasmic tRNA 2-thiolation protein 2 (NCS2) CSB-YP003313SVG
CSB-EP003313SVG
CSB-BP003313SVG
CSB-MP003313SVG
CSB-EP003313SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Conserved oligomeric Golgi complex subunit 5 (COG5) CSB-YP005709SVG
CSB-EP005709SVG
CSB-BP005709SVG
CSB-MP005709SVG
CSB-EP005709SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Monocarboxylate transporter 2 (SLC16A7), partial CSB-YP021414MRG
CSB-EP021414MRG
CSB-BP021414MRG
CSB-MP021414MRG
CSB-EP021414MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant T-complex protein 1 subunit eta CSB-YP004871TLU
CSB-EP004871TLU
CSB-BP004871TLU
CSB-MP004871TLU
CSB-EP004871TLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum V-type proton ATPase catalytic subunit A (vatA), partial CSB-YP002396DKK
CSB-EP002396DKK
CSB-BP002396DKK
CSB-MP002396DKK
CSB-EP002396DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Origin recognition complex subunit 1 (ORC1), partial CSB-YP017231KBK
CSB-EP017231KBK
CSB-BP017231KBK
CSB-MP017231KBK
CSB-EP017231KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Manduca sexta 26S protease regulatory subunit 8 CSB-YP018895MPV
CSB-EP018895MPV
CSB-BP018895MPV
CSB-MP018895MPV
CSB-EP018895MPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Squalus acanthias Solute carrier family 12 member 2 (SLC12A2), partial CSB-YP021383FKW
CSB-EP021383FKW
CSB-BP021383FKW
CSB-MP021383FKW
CSB-EP021383FKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Solute carrier family 12 member 1 (Slc12a1), partial CSB-YP021382RA
CSB-EP021382RA
CSB-BP021382RA
CSB-MP021382RA
CSB-EP021382RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tyrosinase (melC2) CSB-YP025394SOS
CSB-EP025394SOS
CSB-BP025394SOS
CSB-MP025394SOS
CSB-EP025394SOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodiscus sinensis Tyrosinase (TYR) CSB-YP025394PEL
CSB-EP025394PEL
CSB-BP025394PEL
CSB-MP025394PEL
CSB-EP025394PEL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 26S proteasome non-ATPase regulatory subunit 4 (MBP1) CSB-YP018908DOA
CSB-EP018908DOA
CSB-BP018908DOA
CSB-MP018908DOA
CSB-EP018908DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster 40S ribosomal protein S20 (RpS20) CSB-YP020394DLU
CSB-EP020394DLU
CSB-BP020394DLU
CSB-MP020394DLU
CSB-EP020394DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Translation initiation factor IF-3 (infC) CSB-YP015166BRJ
CSB-EP015166BRJ
CSB-BP015166BRJ
CSB-MP015166BRJ
CSB-EP015166BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Callinectes sapidus Metallothionein-1B CSB-YP015108CAS
CSB-EP015108CAS
CSB-BP015108CAS
CSB-MP015108CAS
CSB-EP015108CAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Nucleoside diphosphate kinase (ndk) CSB-YP015889HUV
CSB-EP015889HUV
CSB-BP015889HUV
CSB-MP015889HUV
CSB-EP015889HUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori ATP synthase subunit a (atpB), partial CSB-YP015070HUV1
CSB-EP015070HUV1
CSB-BP015070HUV1
CSB-MP015070HUV1
CSB-EP015070HUV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Ribonuclease 3 (rnc) CSB-YP019809DXP
CSB-EP019809DXP
CSB-BP019809DXP
CSB-MP019809DXP
CSB-EP019809DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis DNA ligase 1 (lig1), partial CSB-YP012930XBE
CSB-EP012930XBE
CSB-BP012930XBE
CSB-MP012930XBE
CSB-EP012930XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila bifasciata NADH-ubiquinone oxidoreductase chain 1 (mt:ND1), partial CSB-YP015076DFS1
CSB-EP015076DFS1
CSB-BP015076DFS1
CSB-MP015076DFS1
CSB-EP015076DFS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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