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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Aspartate carbamoyltransferase (pyrB) CSB-YP809451WAN
CSB-EP809451WAN
CSB-BP809451WAN
CSB-MP809451WAN
CSB-EP809451WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable rRNA maturation factor (WIGBR4470) CSB-YP809452WAN
CSB-EP809452WAN
CSB-BP809452WAN
CSB-MP809452WAN
CSB-EP809452WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein TolB (tolB) CSB-YP809453WAN
CSB-EP809453WAN
CSB-BP809453WAN
CSB-MP809453WAN
CSB-EP809453WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP809454WAN
CSB-EP809454WAN
CSB-BP809454WAN
CSB-MP809454WAN
CSB-EP809454WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable septum site-determining protein MinC (minC) CSB-YP809455WAN
CSB-EP809455WAN
CSB-BP809455WAN
CSB-MP809455WAN
CSB-EP809455WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tyrosine--tRNA ligase (tyrS) CSB-YP809456WAN
CSB-EP809456WAN
CSB-BP809456WAN
CSB-MP809456WAN
CSB-EP809456WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 5'/3'-nucleotidase SurE (surE) CSB-YP809457WAN
CSB-EP809457WAN
CSB-BP809457WAN
CSB-MP809457WAN
CSB-EP809457WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Na (+)/H (+) antiporter nhaA (nhaA), partial CSB-YP809458WAN
CSB-EP809458WAN
CSB-BP809458WAN
CSB-MP809458WAN
CSB-EP809458WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphopantetheine adenylyltransferase (coaD) CSB-YP809459WAN
CSB-EP809459WAN
CSB-BP809459WAN
CSB-MP809459WAN
CSB-EP809459WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Magnesium transport protein CorA (corA), partial CSB-YP809460WAN1
CSB-EP809460WAN1
CSB-BP809460WAN1
CSB-MP809460WAN1
CSB-EP809460WAN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L9 (rplI) CSB-YP809461WAN
CSB-EP809461WAN
CSB-BP809461WAN
CSB-MP809461WAN
CSB-EP809461WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) CSB-YP809462WAN
CSB-EP809462WAN
CSB-BP809462WAN
CSB-MP809462WAN
CSB-EP809462WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosome-binding factor A (rbfA) CSB-YP809463WAN
CSB-EP809463WAN
CSB-BP809463WAN
CSB-MP809463WAN
CSB-EP809463WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP809464WAN
CSB-EP809464WAN
CSB-BP809464WAN
CSB-MP809464WAN
CSB-EP809464WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA gyrase inhibitor YacG (yacG) CSB-YP809465WAN
CSB-EP809465WAN
CSB-BP809465WAN
CSB-MP809465WAN
CSB-EP809465WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leucine--tRNA ligase (leuS), partial CSB-YP809466WAN
CSB-EP809466WAN
CSB-BP809466WAN
CSB-MP809466WAN
CSB-EP809466WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase (arnC), partial CSB-YP809467WAN1
CSB-EP809467WAN1
CSB-BP809467WAN1
CSB-MP809467WAN1
CSB-EP809467WAN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate racemase (murI) CSB-YP809468WAN
CSB-EP809468WAN
CSB-BP809468WAN
CSB-MP809468WAN
CSB-EP809468WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP809469WAN
CSB-EP809469WAN
CSB-BP809469WAN
CSB-MP809469WAN
CSB-EP809469WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L32 (rpmF) CSB-YP809470WAN
CSB-EP809470WAN
CSB-BP809470WAN
CSB-MP809470WAN
CSB-EP809470WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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