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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Iron-sulfur cluster insertion protein ErpA (erpA) CSB-YP809471WAN
CSB-EP809471WAN
CSB-BP809471WAN
CSB-MP809471WAN
CSB-EP809471WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0176 protein WIGBR0650 (WIGBR0650) CSB-YP809472WAN
CSB-EP809472WAN
CSB-BP809472WAN
CSB-MP809472WAN
CSB-EP809472WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S12 (rpsL) CSB-YP809473WAN
CSB-EP809473WAN
CSB-BP809473WAN
CSB-MP809473WAN
CSB-EP809473WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase epsilon chain (atpC) CSB-YP809474WAN
CSB-EP809474WAN
CSB-BP809474WAN
CSB-MP809474WAN
CSB-EP809474WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit delta (atpH) CSB-YP809475WAN
CSB-EP809475WAN
CSB-BP809475WAN
CSB-MP809475WAN
CSB-EP809475WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-aminoethylphosphonate--pyruvate transaminase (phnW) CSB-YP809476VFI
CSB-EP809476VFI
CSB-BP809476VFI
CSB-MP809476VFI
CSB-EP809476VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0397 protein VV2_1534 (VV2_1534), partial CSB-YP809477VFI1
CSB-EP809477VFI1
CSB-BP809477VFI1
CSB-MP809477VFI1
CSB-EP809477VFI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0145 protein VV2_1464 (VV2_1464) CSB-YP809478VFI
CSB-EP809478VFI
CSB-BP809478VFI
CSB-MP809478VFI
CSB-EP809478VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beta-galactosidase (lacZ), partial CSB-YP809479VFI
CSB-EP809479VFI
CSB-BP809479VFI
CSB-MP809479VFI
CSB-EP809479VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyridoxamine kinase (pdxY) CSB-YP809480VFI
CSB-EP809480VFI
CSB-BP809480VFI
CSB-MP809480VFI
CSB-EP809480VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Microbial collagenase (VV2_1146), partial CSB-YP809481VFI
CSB-EP809481VFI
CSB-BP809481VFI
CSB-MP809481VFI
CSB-EP809481VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0251 protein VV2_0946 (VV2_0946) CSB-YP809482VFI
CSB-EP809482VFI
CSB-BP809482VFI
CSB-MP809482VFI
CSB-EP809482VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0265 protein VV2_0759 (VV2_0759) CSB-YP809483VFI
CSB-EP809483VFI
CSB-BP809483VFI
CSB-MP809483VFI
CSB-EP809483VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Periplasmic nitrate reductase (napA), partial CSB-YP809484VFI
CSB-EP809484VFI
CSB-BP809484VFI
CSB-MP809484VFI
CSB-EP809484VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cobyric acid synthase (cobQ) CSB-YP809485VFI
CSB-EP809485VFI
CSB-BP809485VFI
CSB-MP809485VFI
CSB-EP809485VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine dehydrogenase [decarboxylating] (gcvP), partial CSB-YP809486VFI
CSB-EP809486VFI
CSB-BP809486VFI
CSB-MP809486VFI
CSB-EP809486VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaerobic glycerol-3-phosphate dehydrogenase subunit B (glpB) CSB-YP809487VFI
CSB-EP809487VFI
CSB-BP809487VFI
CSB-MP809487VFI
CSB-EP809487VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nucleoid-associated protein VV1_3120 (VV1_3120) CSB-YP809488VFI
CSB-EP809488VFI
CSB-BP809488VFI
CSB-MP809488VFI
CSB-EP809488VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP809489VFI
CSB-EP809489VFI
CSB-BP809489VFI
CSB-MP809489VFI
CSB-EP809489VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor P-like protein (VV1_3058) CSB-YP809490VFI
CSB-EP809490VFI
CSB-BP809490VFI
CSB-MP809490VFI
CSB-EP809490VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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