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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Calomys callosus NADH-ubiquinone oxidoreductase chain 3 (MT-ND3), partial CSB-YP015078CYO1
CSB-EP015078CYO1
CSB-BP015078CYO1
CSB-MP015078CYO1
CSB-EP015078CYO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sigmodon hispidus NADH-ubiquinone oxidoreductase chain 4L (MT-ND4L), partial CSB-YP015080SZK1
CSB-EP015080SZK1
CSB-BP015080SZK1
CSB-MP015080SZK1
CSB-EP015080SZK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scotinomys teguina NADH-ubiquinone oxidoreductase chain 3 (MT-ND3), partial CSB-YP015078SXZ1
CSB-EP015078SXZ1
CSB-BP015078SXZ1
CSB-MP015078SXZ1
CSB-EP015078SXZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onychomys leucogaster NADH-ubiquinone oxidoreductase chain 3 (MT-ND3), partial CSB-YP015078OEK1
CSB-EP015078OEK1
CSB-BP015078OEK1
CSB-MP015078OEK1
CSB-EP015078OEK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peromyscus mexicanus NADH-ubiquinone oxidoreductase chain 3 (MT-ND3), partial CSB-YP015078ETY1
CSB-EP015078ETY1
CSB-BP015078ETY1
CSB-MP015078ETY1
CSB-EP015078ETY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptomys damarensis Cytochrome b (MT-CYB), partial CSB-YP015075DYR
CSB-EP015075DYR
CSB-BP015075DYR
CSB-MP015075DYR
CSB-EP015075DYR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage M1 Single-stranded DNA-binding protein (32) CSB-YP022706ELB
CSB-EP022706ELB
CSB-BP022706ELB
CSB-MP022706ELB
CSB-EP022706ELB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage RB8 Single-stranded DNA-binding protein (32) CSB-YP022706ELO
CSB-EP022706ELO
CSB-BP022706ELO
CSB-MP022706ELO
CSB-EP022706ELO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage RB27 Single-stranded DNA-binding protein (32) CSB-YP022706ELI
CSB-EP022706ELI
CSB-BP022706ELI
CSB-MP022706ELI
CSB-EP022706ELI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fritillaria agrestis 40S ribosomal protein S16 (RPS16) CSB-YP020379FFL
CSB-EP020379FFL
CSB-BP020379FFL
CSB-MP020379FFL
CSB-EP020379FFL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Panax ginseng Superoxide dismutase [Cu-Zn] (SODCC) CSB-YP022397EQW
CSB-EP022397EQW
CSB-BP022397EQW
CSB-MP022397EQW
CSB-EP022397EQW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein N-terminal glutamine amidohydrolase (At2g41760) CSB-YP026083DOA
CSB-EP026083DOA
CSB-BP026083DOA
CSB-MP026083DOA
CSB-EP026083DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ubiquitin-like modifier-activating enzyme 5 (At1g05350) CSB-YP025415DOA
CSB-EP025415DOA
CSB-BP025415DOA
CSB-MP025415DOA
CSB-EP025415DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein mago nashi homolog (At1g02140) CSB-YP013364DOA
CSB-EP013364DOA
CSB-BP013364DOA
CSB-MP013364DOA
CSB-EP013364DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum V-type proton ATPase 16 kDa proteolipid subunit, partial CSB-YP002389SZY1
CSB-EP002389SZY1
CSB-BP002389SZY1
CSB-MP002389SZY1
CSB-EP002389SZY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha (FTA) CSB-YP008777EWE
CSB-EP008777EWE
CSB-BP008777EWE
CSB-MP008777EWE
CSB-EP008777EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP018033HUV
CSB-EP018033HUV
CSB-BP018033HUV
CSB-MP018033HUV
CSB-EP018033HUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori tRNA dimethylallyltransferase (miaA) CSB-YP024529HUV
CSB-EP024529HUV
CSB-BP024529HUV
CSB-MP024529HUV
CSB-EP024529HUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Branched-chain-amino-acid aminotransferase (ilvE) CSB-YP002601HUV
CSB-EP002601HUV
CSB-BP002601HUV
CSB-MP002601HUV
CSB-EP002601HUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter thermautotrophicus Delta-aminolevulinic acid dehydratase (hemB) CSB-YP001558MSR
CSB-EP001558MSR
CSB-BP001558MSR
CSB-MP001558MSR
CSB-EP001558MSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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