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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Ailuropoda melanoleuca Vertnin (VRTN), partial CSB-YP003138AYX
CSB-EP003138AYX
CSB-BP003138AYX
CSB-MP003138AYX
CSB-EP003138AYX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ailuropoda melanoleuca Cyclin-dependent kinase 12 (CDK12), partial CSB-YP005981AYX
CSB-EP005981AYX
CSB-BP005981AYX
CSB-MP005981AYX
CSB-EP005981AYX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ailuropoda melanoleuca Probable polyprenol reductase (SRD5A3), partial CSB-YP022655AYX1
CSB-EP022655AYX1
CSB-BP022655AYX1
CSB-MP022655AYX1
CSB-EP022655AYX1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ailuropoda melanoleuca Fanconi-associated nuclease 1 (FAN1), partial CSB-YP015179AYX
CSB-BP015179AYX
CSB-MP015179AYX
CSB-EP015179AYX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ailuropoda melanoleuca Double-strand-break repair protein rad21-like protein 1 (RAD21L1), partial CSB-YP019258AYX
CSB-EP019258AYX
CSB-BP019258AYX
CSB-MP019258AYX
CSB-EP019258AYX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ailuropoda melanoleuca ORM1-like protein 3 (ORMDL3), partial CSB-YP017241AYX1
CSB-EP017241AYX1
CSB-BP017241AYX1
CSB-MP017241AYX1
CSB-EP017241AYX1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Condylactis gigantea Phospholipase A2 CSB-YP018091DVM
CSB-EP018091DVM
CSB-BP018091DVM
CSB-MP018091DVM
CSB-EP018091DVM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat GTP-binding protein 1 (Gtpbp1) CSB-YP010031RA
CSB-EP010031RA
CSB-BP010031RA
CSB-MP010031RA
CSB-EP010031RA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eubacterium eligens GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582EOW
CSB-EP009582EOW
CSB-BP009582EOW
CSB-MP009582EOW
CSB-EP009582EOW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eubacterium rectale Peptide chain release factor 1 (prfA) CSB-YP015197EOX
CSB-EP015197EOX
CSB-BP015197EOX
CSB-MP015197EOX
CSB-EP015197EOX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Thymidylate kinase (tmk) CSB-YP007226ENU
CSB-EP007226ENU
CSB-BP007226ENU
CSB-MP007226ENU
CSB-EP007226ENU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Cardiolipin synthase (cls), partial CSB-YP005985ENU1
CSB-EP005985ENU1
CSB-BP005985ENU1
CSB-MP005985ENU1
CSB-EP005985ENU1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glucokinase (glk) CSB-YP009319ENU
CSB-EP009319ENU
CSB-BP009319ENU
CSB-MP009319ENU
CSB-EP009319ENU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glycogen debranching enzyme (glgX) CSB-YP001446ENU
CSB-EP001446ENU
CSB-BP001446ENU
CSB-MP001446ENU
CSB-EP001446ENU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Translation initiation factor IF-3 (infC) CSB-YP015166ENU
CSB-EP015166ENU
CSB-BP015166ENU
CSB-MP015166ENU
CSB-EP015166ENU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 6-phosphofructokinase (pfkA) CSB-YP017822ENU
CSB-EP017822ENU
CSB-BP017822ENU
CSB-MP017822ENU
CSB-EP017822ENU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus gammatolerans Flap endonuclease 1 (fen) CSB-YP008585TMP
CSB-EP008585TMP
CSB-BP008585TMP
CSB-MP008585TMP
CSB-EP008585TMP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Edwardsiella ictaluri Thymidylate kinase (tmk) CSB-YP007226EJO
CSB-EP007226EJO
CSB-BP007226EJO
CSB-MP007226EJO
CSB-EP007226EJO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Edwardsiella ictaluri Cysteine desulfurase (iscS) CSB-YP015769EJO
CSB-EP015769EJO
CSB-BP015769EJO
CSB-MP015769EJO
CSB-EP015769EJO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Teredinibacter turnerae Peptide deformylase (def) CSB-YP017707TLR
CSB-EP017707TLR
CSB-BP017707TLR
CSB-MP017707TLR
CSB-EP017707TLR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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