Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rat Tonsoku-like protein (Tonsl), partial CSB-YP015766RA
CSB-EP015766RA
CSB-BP015766RA
CSB-MP015766RA
CSB-EP015766RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Actin filament-associated protein 1-like 1 (Afap1l1) CSB-YP001409RA
CSB-EP001409RA
CSB-BP001409RA
CSB-MP001409RA
CSB-EP001409RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Probable phospholipid-transporting ATPase IIB (Atp9b), partial CSB-YP002423RA
CSB-EP002423RA
CSB-BP002423RA
CSB-MP002423RA
CSB-EP002423RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Inositol monophosphatase 3 (Impad1), partial CSB-YP011697RA1
CSB-EP011697RA1
CSB-BP011697RA1
CSB-MP011697RA1
CSB-EP011697RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Elongation of very long chain fatty acids protein 7 (Elovl7), partial CSB-YP007625RA1
CSB-EP007625RA1
CSB-BP007625RA1
CSB-MP007625RA1
CSB-EP007625RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribosome-recycling factor (frr) CSB-YP014941ENU
CSB-EP014941ENU
CSB-BP014941ENU
CSB-MP014941ENU
CSB-EP014941ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli N-acetyl-D-glucosamine kinase (nagK) CSB-YP015415ENU
CSB-EP015415ENU
CSB-BP015415ENU
CSB-MP015415ENU
CSB-EP015415ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146ENU
CSB-EP019146ENU
CSB-BP019146ENU
CSB-MP019146ENU
CSB-EP019146ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli NAD-dependent malic enzyme (maeA) CSB-YP013633ENU
CSB-EP013633ENU
CSB-BP013633ENU
CSB-MP013633ENU
CSB-EP013633ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribonuclease 3 (rnc) CSB-YP019809ENU
CSB-EP019809ENU
CSB-BP019809ENU
CSB-MP019809ENU
CSB-EP019809ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glycine dehydrogenase [decarboxylating] (gcvP), partial CSB-YP009493ENU
CSB-EP009493ENU
CSB-BP009493ENU
CSB-MP009493ENU
CSB-EP009493ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Agmatinase (speB) CSB-YP001447ENU
CSB-EP001447ENU
CSB-BP001447ENU
CSB-MP001447ENU
CSB-EP001447ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus gammatolerans Serine hydroxymethyltransferase (glyA) CSB-YP021274TMP
CSB-EP021274TMP
CSB-BP021274TMP
CSB-MP021274TMP
CSB-EP021274TMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus gammatolerans tRNA pseudouridine synthase A (truA) CSB-YP019086TMP
CSB-EP019086TMP
CSB-BP019086TMP
CSB-MP019086TMP
CSB-EP019086TMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Edwardsiella ictaluri Acyl carrier protein (acpP) CSB-YP015636EJO
CSB-EP015636EJO
CSB-BP015636EJO
CSB-MP015636EJO
CSB-EP015636EJO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Edwardsiella ictaluri Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917EJO
CSB-EP022917EJO
CSB-BP022917EJO
CSB-MP022917EJO
CSB-EP022917EJO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Edwardsiella ictaluri Nucleoside diphosphate kinase (ndk) CSB-YP015889EJO
CSB-EP015889EJO
CSB-BP015889EJO
CSB-MP015889EJO
CSB-EP015889EJO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Edwardsiella ictaluri Methionyl-tRNA formyltransferase (fmt) CSB-YP015150EJO
CSB-EP015150EJO
CSB-BP015150EJO
CSB-MP015150EJO
CSB-EP015150EJO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Edwardsiella ictaluri Translation initiation factor IF-2 (infB), partial CSB-YP015164EJO
CSB-EP015164EJO
CSB-BP015164EJO
CSB-MP015164EJO
CSB-EP015164EJO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Edwardsiella ictaluri Lipoyl synthase (lipA) CSB-YP012927EJO
CSB-EP012927EJO
CSB-BP012927EJO
CSB-MP012927EJO
CSB-EP012927EJO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>