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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Saguinus mystax Membrane cofactor protein (CD46) CSB-YP004939SWA
CSB-EP004939SWA
CSB-BP004939SWA
CSB-MP004939SWA
CSB-EP004939SWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Antechinomys laniger Cytochrome b (MT-CYB), partial CSB-YP015075BZS
CSB-EP015075BZS
CSB-BP015075BZS
CSB-MP015075BZS
CSB-EP015075BZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ctenomys leucodon Cytochrome b (MT-CYB), partial CSB-YP015075DZB
CSB-EP015075DZB
CSB-BP015075DZB
CSB-MP015075DZB
CSB-EP015075DZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dasyurus geoffroii Cytochrome b (MT-CYB), partial CSB-YP015075DIP
CSB-EP015075DIP
CSB-BP015075DIP
CSB-MP015075DIP
CSB-EP015075DIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptomys choma Cytochrome b (MT-CYB), partial CSB-YP015075DYQ
CSB-EP015075DYQ
CSB-BP015075DYQ
CSB-MP015075DYQ
CSB-EP015075DYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tragelaphus imberbis Cytochrome b (MT-CYB), partial CSB-YP015075TPD
CSB-EP015075TPD
CSB-BP015075TPD
CSB-MP015075TPD
CSB-EP015075TPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Branchiostoma lanceolatum ATP synthase subunit a (ATP6), partial CSB-YP015070BVZ1
CSB-EP015070BVZ1
CSB-BP015070BVZ1
CSB-MP015070BVZ1
CSB-EP015070BVZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Cytochrome c oxidase subunit 3 (cox3), partial CSB-YP015074DKK1
CSB-EP015074DKK1
CSB-BP015074DKK1
CSB-MP015074DKK1
CSB-EP015074DKK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Myxine glutinosa Cytochrome c oxidase subunit 1 (MT-CO1), partial CSB-YP015072MWW
CSB-EP015072MWW
CSB-BP015072MWW
CSB-MP015072MWW
CSB-EP015072MWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetotheres cachinnans Cytochrome b (MT-CYB), partial CSB-YP015075HWD
CSB-EP015075HWD
CSB-BP015075HWD
CSB-MP015075HWD
CSB-EP015075HWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caracara plancus Cytochrome b (MT-CYB), partial CSB-YP015075DQF
CSB-EP015075DQF
CSB-BP015075DQF
CSB-MP015075DQF
CSB-EP015075DQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dasypus novemcinctus NADH-ubiquinone oxidoreductase chain 1 (MT-ND1), partial CSB-YP015076DIO
CSB-EP015076DIO
CSB-BP015076DIO
CSB-MP015076DIO
CSB-EP015076DIO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dasypus novemcinctus Cytochrome c oxidase subunit 3 (MT-CO3), partial CSB-YP015074DIO1
CSB-EP015074DIO1
CSB-BP015074DIO1
CSB-MP015074DIO1
CSB-EP015074DIO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dasypus novemcinctus NADH-ubiquinone oxidoreductase chain 6 (MT-ND6), partial CSB-YP015082DIO1
CSB-EP015082DIO1
CSB-BP015082DIO1
CSB-MP015082DIO1
CSB-EP015082DIO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Struthio camelus Cytochrome c oxidase subunit 1 (MT-CO1), partial CSB-YP015072FOU
CSB-EP015072FOU
CSB-BP015072FOU
CSB-MP015072FOU
CSB-EP015072FOU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Struthio camelus NADH-ubiquinone oxidoreductase chain 4L (MT-ND4L), partial CSB-YP015080FOU1
CSB-EP015080FOU1
CSB-BP015080FOU1
CSB-MP015080FOU1
CSB-EP015080FOU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scyliorhinus canicula NADH-ubiquinone oxidoreductase chain 2 (MT-ND2), partial CSB-YP015077SYA
CSB-EP015077SYA
CSB-BP015077SYA
CSB-MP015077SYA
CSB-EP015077SYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorex sadonis Cytochrome b (MT-CYB), partial CSB-YP015075FJL1
CSB-EP015075FJL1
CSB-BP015075FJL1
CSB-MP015075FJL1
CSB-EP015075FJL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oxymycterus rufus NADH-ubiquinone oxidoreductase chain 4L (MT-ND4L), partial CSB-YP015080OFZ1
CSB-EP015080OFZ1
CSB-BP015080OFZ1
CSB-MP015080OFZ1
CSB-EP015080OFZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nyctomys sumichrasti NADH-ubiquinone oxidoreductase chain 4L (MT-ND4L), partial CSB-YP015080NID1
CSB-EP015080NID1
CSB-BP015080NID1
CSB-MP015080NID1
CSB-EP015080NID1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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